5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium
Based on 1 Customer Validation
Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is commonly used as a substrate for phosphatase assays in biochemistry and molecular biology. This compound is converted by phosphatases to a yellow compound that can be easily measured spectrophotometrically or fluorometrically. Furthermore, it has been used as a useful reagent in enzyme-catalyzed reactions and a tool for studying signal transduction pathways.
For research use only. We do not sell to patients.
- Purity : 99.95%
- CAS No.: 102185-33-1
- Formula: C8H4BrClNNa2O4P
- Molecular Weight:370.43
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
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HY-15928
4-Nitrophenyl phosphate disodium -
HY-15934
X-GAL -
HY-15922
Luminol -
HY-DY2002
X-GAL (solution) -
HY-15930
TMB -
HY-15924
Thiazolyl Blue -
HY-W010737
Guanosine triphosphate disodium -
HY-15915
DTNB -
HY-15909
BCIP -
HY-137779
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside -
HY-W028393
Indole-3-pyruvic acid -
HY-15930A
TMB dihydrochloride
Biological Activity
Description
In Vitro
Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 102185-33-1
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Appearance Solid
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Molecular Weight 370.43
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Formula C8H4BrClNNa2O4P
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Color White to off-white
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SMILES
ClC1=C(Br)C=CC2=C1C(OP(O[Na])(O[Na])=O)=CN2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
H2O : 41.67 mg/mL (112.49 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (247 KB)
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SDS (769 KB)
- English - EN (769 KB)
- Français - FR (769 KB)
- Deutsch - DE (769 KB)
- Norwegian - NO (769 KB)
- Español - ES (769 KB)
- Swedish - SV (769 KB)
- Italian - IT (769 KB)
- Korean - KR (769 KB)
- Portuguese - PT (769 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.6996 mL | 13.4978 mL | 26.9957 mL | 67.4891 mL |
| 5 mM | 0.5399 mL | 2.6996 mL | 5.3991 mL | 13.4978 mL | |
| 10 mM | 0.2700 mL | 1.3498 mL | 2.6996 mL | 6.7489 mL | |
| 15 mM | 0.1800 mL | 0.8999 mL | 1.7997 mL | 4.4993 mL | |
| 20 mM | 0.1350 mL | 0.6749 mL | 1.3498 mL | 3.3745 mL | |
| 25 mM | 0.1080 mL | 0.5399 mL | 1.0798 mL | 2.6996 mL | |
| 30 mM | 0.0900 mL | 0.4499 mL | 0.8999 mL | 2.2496 mL | |
| 40 mM | 0.0675 mL | 0.3374 mL | 0.6749 mL | 1.6872 mL | |
| 50 mM | 0.0540 mL | 0.2700 mL | 0.5399 mL | 1.3498 mL | |
| 60 mM | 0.0450 mL | 0.2250 mL | 0.4499 mL | 1.1248 mL | |
| 80 mM | 0.0337 mL | 0.1687 mL | 0.3374 mL | 0.8436 mL | |
| 100 mM | 0.0270 mL | 0.1350 mL | 0.2700 mL | 0.6749 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium Related Classifications
HY-W045537 Related Classifications
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