H-Ala-pNA 

H-Ala-pNA is an L-amino acid p-nitroaniline (pNA) derivative and a specific substrate for leukotriene A4 hydrolase. The D-enantiomer of H-Ala-pNA shows no activity toward leukotriene A4 hydrolase. H-Ala-pNA can be catalytically hydrolyzed by leukotriene A4 hydrolase, and the p-nitroaniline produced during the reaction is monitored spectrophotometrically at 405 nm to enable quantitative detection of enzyme activity. H-Ala-pNA is used to evaluate the potency of inhibitors targeting the amidase activity of leukotriene A4 hydrolase^[1].

H-Ala-pNA (1.5 mM) is hydrolyzed by purified human leukocyte leukotriene A₄ hydrolase with a K_m of 500 μM, kcat of 6.0×10^-1 s^-1, Vmax of 530 nmol/min per mg, and kcat/K_m of 1.2×10³ M^-1s^-1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

209.20

C₉H₁₁N₃O₃

1668-13-9

C[C@H](N)C(NC1=CC=C(C=C1)N(=O)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yuan W, et al. Development of selective tight-binding inhibitors of leukotriene A4 hydrolase. J Med Chem. 1993;36(2):211-220.  [Content Brief]