MK-28
Based on 9 publication(s) in Google Scholar
MK-28 is a potent and selective PERK activator. MK-28 exhibits remarkable pharmacokinetic properties and high BBB penetration in mice.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 864388-65-8
- Formula: C24H20N4O2
- Molecular Weight:396.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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CNS-Penetrant Compound Library
HY-L028
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Anti-Aging Compound Library
HY-L034
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Endoplasmic Reticulum Stress Compound Library
HY-L054
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Heterocyclic Compound Library
HY-L138
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Highly Selective Activators Library
HY-L159
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) MK-28
More- Signal Transduct Target Ther. 2025 Dec 17;10(1):413. [Abstract]
- Cancer Commun (Lond). 2025 Nov 10. [Abstract]
- Nat Aging. 2024 Jun;4(6):839-853. [Abstract]
- Cell Mol Life Sci. 2025 Aug 25;82(1):318. [Abstract]
- PLoS One. 2023 May 18;18(5):e0283943. [Abstract]
- bioRxiv. 2025 Aug 22:2025.08.18.670862. [Abstract]
- Patent. US20240269136A1.
- bioRxiv. 2023 Sep 1:2023.08.31.555821. [Abstract]
- bioRxiv. 2023 May 17:2023.03.11.532186. [Abstract]
Customer Validation & Images
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IF
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WB
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RT-PCR
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Histological Imaging/Staining
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Bio/Physico-chemical Assay
Biological Activity
MK-28 (0-100 μM) shows PERK selectivity in vitro on a 391-kinase panel and rescues cells (but not PERK / cells) from ER stress-induced apoptosis[1].
ATF4 protein levels are increased signifcantly, up to 2.5-fold, in STHdhQ111/111 cells at high MK-28 concentration. CHOP and GADD34 mRNA levels show a signifcant increase in both cell types, up to 10- and 5-fold respectively[1].
MK-28 has little or no efect on EIF2AK1 (HRI) or EIF2AK2 (PKR), but it activats EIF2AK4 (GCN2)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:STHdhQ111/111 cells.
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Concentration:0-100 μM.
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Incubation Time:48 h.
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Result:Rescued cells from ER stress-induced apoptosis.
MK-28 (1 mg/kg, IP, daily for 28 days) improves systemic function and survival in R6/2 mice and induces increased levels of eIF2α-P in the mouse brain striatum[1].
MK-28 (Transient subcutaneous delivery) significantly improves motor and executive functions and delayed death onset in R6/2 mice, showing no toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:R6/2 mice[1].
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Dosage:1 mg/kg.
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Administration:IP, daily for 28 days.
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Result:Incerased the survival.
Chemical Information
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CAS No. 864388-65-8
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Appearance Solid
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Molecular Weight 396.44
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Formula C24H20N4O2
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Color Light yellow to yellow
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SMILES
OC1=CC=C(C=C1O)/C=N/N(C2=NC(C3=CC=CC=C3)=CC(C4=CC=CC=C4)=N2)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (9)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Disruption of heme homeostasis by nuclear receptor Nur77 induces pyroptosis through granzyme B-dependent GSDMC cleavage. [Abstract]2025 Dec 17;10(1):413. PMID: 41407678 -
Cancer Commun (Lond)
Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3-dependent PDGFB signaling. [Abstract]2025 Nov 10. PMID: 41212905 -
Nat Aging
Estrogen counteracts age-related decline in beige adipogenesis through the NAMPT-regulated ER stress response. [Abstract]2024 Jun;4(6):839-853. PMID: 38858606
MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]
Representative images of UCP1 immunofluorescence staining. SVF cells isolated from iWAT of 2-month-old male mice were pre-treated with either vehicle, E2 (10 nM), or three different activators of ER stress MK-28, IXA4, AA147 (10 μM) before differentiating into beige adipocytes.
MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]
Western blot analysis of PERK and PERK phosphorylation in iWAT from vehicle and MK-28 (10 μM).
MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]
qPCR analysis of thermogenic gene expression treated with MK-28 (10 μM).
MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]
Representative images of H&E staining in the middle part of iWAT treated with MK-28 (1 mg/kg, i.p.).
MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]
The blood glucose levels of the vehicle or MK28 (1 mg/kg, i.p.) treated group in 2-month-old male mice after intraperitoneal injection of glucose tolerance test and Area under curve (AUC) analysis by one way-ANOVA followed by Tukey’s post-hoc test.
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Cell Mol Life Sci
RCN1 downregulation-driven endoplasmic reticulum stress impairs endothelial function and diabetic foot ulcer healing. [Abstract]2025 Aug 25;82(1):318. PMID: 40853392 -
PLoS One
Development of a cell-free screening assay for the identification of direct PERK activators. [Abstract]2023 May 18;18(5):e0283943. PMID: 37200357 -
bioRxiv
Ceramide-induced Endoplasmic Reticulum Stress as a Targetable Vulnerability in Endocrine Therapy-Resistant Breast Cancer. [Abstract]2025 Aug 22:2025.08.18.670862. PMID: 40894774 -
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bioRxiv
Estrogen prevents age-dependent beige adipogenesis failure through NAMPT-controlled ER stress pathway. [Abstract]2023 Sep 1:2023.08.31.555821. PMID: 37693431 -
bioRxiv
Pharmacologic Activation of a Compensatory Integrated Stress Response Kinase Promotes Mitochondrial Remodeling in PERK-deficient Cells. [Abstract]2023 May 17:2023.03.11.532186. PMID: 36945406
Solvent & Solubility
DMSO : 12.5 mg/mL (31.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.25 mg/mL (3.15 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.5224 mL | 12.6122 mL | 25.2245 mL | 63.0612 mL |
| 5 mM | 0.5045 mL | 2.5224 mL | 5.0449 mL | 12.6122 mL | |
| 10 mM | 0.2522 mL | 1.2612 mL | 2.5224 mL | 6.3061 mL | |
| 15 mM | 0.1682 mL | 0.8408 mL | 1.6816 mL | 4.2041 mL | |
| 20 mM | 0.1261 mL | 0.6306 mL | 1.2612 mL | 3.1531 mL | |
| 25 mM | 0.1009 mL | 0.5045 mL | 1.0090 mL | 2.5224 mL | |
| 30 mM | 0.0841 mL | 0.4204 mL | 0.8408 mL | 2.1020 mL |
MK-28 Related Classifications
HY-137207 Related Classifications
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