2. Cell Cycle/DNA Damage
  3. IRE1
  4. IXA4

IXA4 is a highly selective, non-toxic IRE1/XBP1s activator. IXA4 activates IRE1/XBP1s signaling without globally activating the unfolded protein response (UPR) or other stress-responsive signaling pathways (e.g., the heat shock response or oxidative stress response). IXA4 reduces secretion of APP through IRE1 activation.

For research use only. We do not sell to patients.

CAS No. : 1185329-96-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 9 publication(s) in Google Scholar

IXA4 Related Antibodies

Top Publications Citing Use of Products

    IXA4 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 May 14:298:118321.  [Abstract]

    Survival rates and hatching rates of zebrafish embryos exposed to Tl (200 μg/L) with or without IXA4 (20 μM) treatments.

    IXA4 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 May 14:298:118321.  [Abstract]

    IXA4 (20 μM; 6-48 hpf) treatment significantly restored the expression of NER-related genes (xpc, rad23b, and ercc5) and markedly increased the expression of atm in zebrafish embryos.

    IXA4 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 May 14:298:118321.  [Abstract]

    The representative figures of H&E-stained sections of zebrafish midbrain, hindbrain, and heart transverse after being treated with Tl (800 μg/L) and Tl + IXA4 (20 μM) from 6 to 72 hpf. IXA4 offered protective benefits against the histological alterations induced by Tl in both cerebral and cardiac tissues.

    IXA4 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Nov 17;14(1):7441.  [Abstract]

    PBMCs obtained from influenza patients with cytokine storm were pretreated with IXA4 (10 μM; 6 h). The expression of iNOS, CD80, and CD86 was detected. IXA4 significantly inhibited M1-like hyperpolarization in PBMCs.

    IXA4 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Nov 17;14(1):7441.  [Abstract]

    IXA4 (10 μM; 6 h) effectively alleviated the outburst of proinflammatory cytokines in influenza patients.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    IXA4 is a highly selective, non-toxic IRE1/XBP1s activator. IXA4 activates IRE1/XBP1s signaling without globally activating the unfolded protein response (UPR) or other stress-responsive signaling pathways (e.g., the heat shock response or oxidative stress response). IXA4 reduces secretion of APP through IRE1 activation[1].

    In Vitro

    IXA4 (10 μM; 4 hours) selectively upregulates XBP1s mRNA, relative to genes regulated by ATF6 (e.g., BiP) or PERK (e.g., CHOP), in other cell lines including Huh7 and SHSY5Y cells[1].
    IXA4 (10μM; 18 hours) reduces Aβ levels 50% in conditioned media prepared on CHO7PA2 cells expressing the V717F APP (APPV717F) mutant[1].
    IXA4 rescues mitochondrial defects in SH-SY5Y cells expressing disease-relevant APP mutants. IXA4 (10μM; 4 hours) promotes adaptive IRE1/XBP1s signaling, but not RIDD, following 4 hrs of treatment in HEK293T cells[1].
    IXA4 also promotes selective transcriptional remodeling of ER proteostasis pathways, relative to cytosolic or mitochondrial pathways[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    IXA4 Related Antibodies
    Molecular Weight

    436.50

    Formula

    C24H28N4O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N(C)CCOC1=CC=C(C)C=C1)CN2N=CC(NC(CCOC3=CC=CC=C3)=O)=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (229.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2910 mL 11.4548 mL 22.9095 mL
    5 mM 0.4582 mL 2.2910 mL 4.5819 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 5 mg/mL (11.45 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.92%

    SDS (394 KB)

    COA (245 KB) HNMR (265 KB) RP-HPLC (257 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2910 mL 11.4548 mL 22.9095 mL 57.2738 mL
    5 mM 0.4582 mL 2.2910 mL 4.5819 mL 11.4548 mL
    10 mM 0.2291 mL 1.1455 mL 2.2910 mL 5.7274 mL
    15 mM 0.1527 mL 0.7637 mL 1.5273 mL 3.8183 mL
    20 mM 0.1145 mL 0.5727 mL 1.1455 mL 2.8637 mL
    25 mM 0.0916 mL 0.4582 mL 0.9164 mL 2.2910 mL
    30 mM 0.0764 mL 0.3818 mL 0.7637 mL 1.9091 mL
    40 mM 0.0573 mL 0.2864 mL 0.5727 mL 1.4318 mL
    50 mM 0.0458 mL 0.2291 mL 0.4582 mL 1.1455 mL
    60 mM 0.0382 mL 0.1909 mL 0.3818 mL 0.9546 mL
    80 mM 0.0286 mL 0.1432 mL 0.2864 mL 0.7159 mL
    100 mM 0.0229 mL 0.1145 mL 0.2291 mL 0.5727 mL
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    IXA4 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    IXA41185329-96-7IXA 4IXA-4IRE1Inositol requiring enzyme 1unfolded protein responseUPRendoplasmic reticulum (ER) proteostasisprotein secretionInhibitorinhibitorinhibit

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