2. Metabolic Enzyme/Protease Apoptosis
  3. Phosphatase Apoptosis
  4. BTdCPU

BTdCPU is a potent heme regulated inhibitor kinase (HRI) activator that promotes eIF2α phosphorylation and induces apoptosis in Dexamethasone (HY-14648) (Dex)-resistant cancer cells. BTdCPU can be used in the study of cancers such as multiple myeloma and Dex-resistant multiple myeloma.

For research use only. We do not sell to patients.

BTdCPU Chemical Structure

BTdCPU Chemical Structure

CAS No. : 1257423-87-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 423 In-stock
Solution
10 mM * 1 mL in DMSO USD 423 In-stock
Solid
1 mg USD 183 In-stock
5 mg USD 385 In-stock
10 mg USD 605 In-stock
25 mg USD 1089 In-stock
50 mg USD 1815 In-stock
100 mg USD 2475 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

BTdCPU Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BTdCPU

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BTdCPU is a potent heme regulated inhibitor kinase (HRI) activator that promotes eIF2α phosphorylation and induces apoptosis in Dexamethasone (HY-14648) (Dex)-resistant cancer cells. BTdCPU can be used in the study of cancers such as multiple myeloma and Dex-resistant multiple myeloma[1][2].

IC50 & Target

HRI[1][2].
In Vitro

BTdCPU (10 μM; 4, 8 h) BTdCPU induces phosphorylation of eIF2α and promotes cell death in MM cells[1].
BTdCPU (10 μM; 4, 8 h) shows cytotoxic for dex-resistant primary MM cells with relative sparing of normal cells perhaps[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BTdCPU Related Antibodies

Western Blot Analysis[1]

Cell Line: MM1.S and MM1.R cells
Concentration: 10 μM
Incubation Time: 0, 4, 8 h
Result: Induced phosphorylation of eIF2α and upregulated mRNA and protein levels of the pro-apoptotic protein CHOP.

Western Blot Analysis[1]

Cell Line: Bone marrow mononuclear cells (MNC) from multiple myeloma (MM) patient or healthy donor
Concentration: 10 μM
Incubation Time: 0, 4, 8 h
Result: Induced early expression of CHOP in MM patient cells, but not in healthy bone marrow mononuclear cells (MNC).
In Vivo

BTdCPU (175 mg/kg; i.p.; single daily for 21 days) inhibits tumor growth without toxicity in mice xenograft breast tumors model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female nude mice (mice xenograft breast tumors model)[2].
Dosage: 175 mg/kg
Administration: Intraperitoneal injection; single daily for 21 days
Result: Led to complete tumor stasis,which persisted for the remainder of the 3-week study
Showed good safety in mice.
Molecular Weight

339.20

Formula

C13H8Cl2N4OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C2N=NSC2=C1)NC3=CC=C(C(Cl)=C3)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (368.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9481 mL 14.7406 mL 29.4811 mL
5 mM 0.5896 mL 2.9481 mL 5.8962 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.17 mg/mL (6.40 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (6.13 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.15%

COA (246 KB) LCMS (261 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9481 mL 14.7406 mL 29.4811 mL 73.7028 mL
5 mM 0.5896 mL 2.9481 mL 5.8962 mL 14.7406 mL
10 mM 0.2948 mL 1.4741 mL 2.9481 mL 7.3703 mL
15 mM 0.1965 mL 0.9827 mL 1.9654 mL 4.9135 mL
20 mM 0.1474 mL 0.7370 mL 1.4741 mL 3.6851 mL
25 mM 0.1179 mL 0.5896 mL 1.1792 mL 2.9481 mL
30 mM 0.0983 mL 0.4914 mL 0.9827 mL 2.4568 mL
40 mM 0.0737 mL 0.3685 mL 0.7370 mL 1.8426 mL
50 mM 0.0590 mL 0.2948 mL 0.5896 mL 1.4741 mL
60 mM 0.0491 mL 0.2457 mL 0.4914 mL 1.2284 mL
80 mM 0.0369 mL 0.1843 mL 0.3685 mL 0.9213 mL
100 mM 0.0295 mL 0.1474 mL 0.2948 mL 0.7370 mL
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BTdCPU Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BTdCPU1257423-87-2PhosphataseApoptosisMM1.SMM1.Rcancerdexamethasone resistancemultiple myelomaInhibitorinhibitorinhibit

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