2. Anti-infection Cytoskeleton Epigenetics Cell Cycle/DNA Damage Immunology/Inflammation TGF-beta/Smad Stem Cell/Wnt MAPK/ERK Pathway
  3. Influenza Virus Collagen Sirtuin Interleukin Related TGF-beta/Smad ERK p38 MAPK
  4. Tetramethyluric acid

Tetramethyluric acid  (Synonyms: 1,3,7,9-Tetramethyluric acid; Theacrine)

Cat. No.: HY-119575 Purity: 99.63%
COA
SDS
Handling Instructions Technical Support

Tetramethyluric acid (1,3,7,9-Tetramethyluric acid) is an orally active purine alkaloid in Coffea species and Camellia kucha. Tetramethyluric acid exhibits antiviral, and anti-inflammatory activity. Tetramethyluric acid can be used for the research of inflammation disease, and influenza virus infection.

For research use only. We do not sell to patients.

Tetramethyluric acid

Tetramethyluric acid Chemical Structure

CAS No. : 2309-49-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Solution
10 mM * 1 mL in Water In-stock
Solid
25 g In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Tetramethyluric acid Related Antibodies

Top Publications Citing Use of Products

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Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7 SIRT4

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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ERK ERK1 ERK2 ERK5 ERK8

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tetramethyluric acid (1,3,7,9-Tetramethyluric acid) is an orally active purine alkaloid in Coffea species and Camellia kucha. Tetramethyluric acid exhibits antiviral, and anti-inflammatory activity. Tetramethyluric acid can be used for the research of inflammation disease, and influenza virus infection[1][2][3][4][5].

IC50 & Target[1]

SIRT3

 

IL-6

 

IL-1β

 

In Vitro

Tetramethyluric acid (50-100 μM) exhibits anti-influenza virus activity in MDCK cells, inhibiting NS1 protein expression[1].
Tetramethyluric acid (1.5×10-2 M) reduces 32P-labelled orthophosphate incorporation into DNA and RNA and increases ATP levels by 40% in excised Vicia faba root tips[2].
Tetramethyluric acid (2×10-2 M; 120 min) induces chromosomal aberrations in Vicia faba root tips[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tetramethyluric acid Related Antibodies
In Vivo

Tetramethyluric acid (10 mg/kg; p.o.) upregulates collagen II, SIRT3, FOXO3a, and SOD2 expression to improve intervertebral disc degeneration in mice[1].
Tetramethyluric acid (20 mg/kg; i.g.) improves cardiac function in ovariectomized mice with myocardial infarction by activating SIRT3 and reducing myocardial fibrosis and apoptosis[1].
Tetramethyluric acid (8-32 mg/kg; p.o.; single dose) significantly inhibits Xylene-induced ear edema and λ-Carrageenan (HY-N9470)-induced hind paw edema[1].
Tetramethyluric acid (10-30 mg/kg; p.o.; daily; 7 days) reduces hepatic inflammation and liver damage in restraint-stressed mice[1].
Tetramethyluric acid (12.5-25.0 mg/kg; p.o.) attenuates FIA-induced rheumatoid arthritis in SD rats via the TGF-β/SMAD pathway[1].
Tetramethyluric acid (24-48 mg/kg; i.p.; single pre-treatment dose) significantly attenuates adenosine receptor agonist-induced motor depression in Long Evans rats via adenosergic and dopaminergic systems[1].
Tetramethyluric acid (5-15 mg/kg; p.o.) reverses restraint stress-induced learning and memory impairment in male Kunming mice[1].
Tetramethyluric acid (3-30 mg/kg; i.g.) produces antidepressant effects in male NIH mice by modulating monoamine neurotransmitters[1].
Tetramethyluric acid (10-20 mg/kg; p.o.) ameliorates HFD-induced NAFLD in C57BL/6 mice via the SIRT3/LCAD signaling pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice intervertebral disc degeneration model[1]
Dosage: 10 mg/kg
Administration: p.o.
Result: Stimulated the expression of collagen II, SIRT3, FOXO3a, and SOD2.
Animal Model: Estrogen-deficient mice[1]
Dosage: 20 mg/kg
Administration: i.g.
Result: Activated SIRT3, repressed myocardial fibrosis and apoptosis, and improved cardiac function.
Animal Model: Mice with ear and hind paw edema induced by Xylene and λ-Carrageenan[1]
Dosage: 8; 16; 32 mg/kg
Administration: p.o.; single dose
Result: Inhibited Xylene-induced ear edema with inhibition percentages of 10.1%, 12.9%, and 45.2% at 8, 16, and 32 mg/kg respectively.
Achieved maximal anti-edema effects at 2 hours post-induction of 14.1%, 16.6%, and 20.1% at 8, 16, and 32 mg/kg, respectively.
Inhibited λ-Carrageenan-induced hind paw edema in a dose-dependent manner.
Animal Model: Restraint-stressed mice[1]
Dosage: 10; 20; 30 mg/kg
Administration: p.o.; daily; 7 days
Result: Reduced hepatic mRNA levels of IL-6, IL-1β, TNF-α, and IFN-γ.
Decreased plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels.
Reversed histologic liver damage.
Animal Model: Sprague-Dawley (SD) rats with rheumatoid arthritis induced by FIA[1]
Dosage: 12.5; 25.0 mg/kg
Administration: p.o.
Result: Attenuated rheumatoid arthritis by suppressing IL-6 and activating TGF-β via the TGF-β/SMAD pathway.
Upregulated RNA and protein expression of SMAD3, p-ERK, and p-p38.
Downregulated nuclear factor-kB (NF-kB) and IL-6 expression.
Animal Model: Long Evans rats[1]
Dosage: 24; 48 mg/kg
Administration: i.p.; single pre-treatment dose
Result: Significantly attenuated motor depression induced by adenosine receptor agonists, an effect reduced by D1R and D2R antagonists.
Animal Model: Male Kunming mice[1]
Dosage: 5; 10; 15 mg/kg
Administration: p.o.
Result: Significantly reversed learning and memory impairment by normalizing glucose metabolism, restoring 5-HT levels, and inhibiting phosphodiesterase in the brain.
Animal Model: NIH mice (male)[1]
Dosage: 3; 10; 30 mg/kg
Administration: i.g.
Result: Shortened immobility time in the tail suspension test.
Enhanced rotatory locomotor activities during forced swimming test.
Increased mortality induced by yohimbine and head-twitching number induced by 5-HTP.
Improved reserpine-induced hypothermia, akinesia, and eye ptosis.
Animal Model: C57BL/6 mice[1]
Dosage: 10; 20 mg/kg
Administration: p.o.
Result: Inhibited hepatic steatosis and liver inflammation.
Improved energy expenditure.
Ameliorated acylcarnitine metabolism disorder via the SIRT3/LCAD signaling pathway.
Clinical Trial
Molecular Weight

224.22

Formula

C9H12N4O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C2=C(N(C)C(N2C)=O)N(C)C(N1C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (111.50 mM; Need ultrasonic)

DMSO : 5 mg/mL (22.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4599 mL 22.2995 mL 44.5991 mL
5 mM 0.8920 mL 4.4599 mL 8.9198 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.63%

SDS (392 KB)

COA (251 KB) HNMR (154 KB) LCMS (102 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.4599 mL 22.2995 mL 44.5991 mL 111.4976 mL
5 mM 0.8920 mL 4.4599 mL 8.9198 mL 22.2995 mL
10 mM 0.4460 mL 2.2300 mL 4.4599 mL 11.1498 mL
15 mM 0.2973 mL 1.4866 mL 2.9733 mL 7.4332 mL
20 mM 0.2230 mL 1.1150 mL 2.2300 mL 5.5749 mL
H2O 25 mM 0.1784 mL 0.8920 mL 1.7840 mL 4.4599 mL
30 mM 0.1487 mL 0.7433 mL 1.4866 mL 3.7166 mL
40 mM 0.1115 mL 0.5575 mL 1.1150 mL 2.7874 mL
50 mM 0.0892 mL 0.4460 mL 0.8920 mL 2.2300 mL
60 mM 0.0743 mL 0.3717 mL 0.7433 mL 1.8583 mL
80 mM 0.0557 mL 0.2787 mL 0.5575 mL 1.3937 mL
100 mM 0.0446 mL 0.2230 mL 0.4460 mL 1.1150 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tetramethyluric acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetramethyluric acid2309-49-11,3,7,9-Tetramethyluric acid TheacrineInfluenza VirusCollagenSirtuinInterleukin RelatedTGF-beta/SmadERKp38 MAPKILTransforming growth factor betaExtracellular signal regulated kinasesadenosine A1/A2A receptorsNF-κBCamellia assamica var. kucha3T3-L1 cellsSMAD3AMPKSIRT3Coffea speciesIL-6ACCInhibitorinhibitorinhibit

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