TP4 (Nile tilapia piscidin)

Name: TP4 (Nile tilapia piscidin)
Brand: MedChemExpress
SKU: HY-P5557
Rating: 4.5 (1 reviews)

Cat. No.: HY-P5557 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
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TP4 (Nile tilapia piscidin) is an orally active amphipathic α-helical cationic antimicrobial peptide. TP4 (Nile tilapia piscidin) exerts broad-spectrum bactericidal activity against Gram-positive and Gram-negative bacteria by disrupting cell membrane structures, and also exhibits certain hemolytic activity. TP4 (Nile tilapia piscidin) regulates the innate immune system and enhances the expression of lysozymes, cytokines and antioxidant enzymes to resist bacterial infection. TP4 (Nile tilapia piscidin) also stimulates the proliferation of keratinocytes and fibroblasts, and significantly promotes the healing of wounds infected with MRSA. TP4 (Nile tilapia piscidin) can be used in research related to bacterial infection, peritonitis, wound healing and other fields.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

TP4 (Nile tilapia piscidin) Chemical Structure

CAS No. : 1429184-62-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

TP4 (Nile tilapia piscidin) (10-30 μg/mL; 24 h) inhibits the proliferation of tilapia ovary (TO2) cells in vitro in a dose-dependent manner, and reduces the cell viability to approximately 35% at a concentration of 28 μg/mL^[1].
TP4 (Nile tilapia piscidin) (0.3125-40 μg/mL; 48 h) promotes the proliferation of Hs-68 human fibroblasts at concentrations of 2.5, 5, and 10 μg/mL, and does not reduce cell viability at these concentrations^[2].
TP4 (Nile tilapia piscidin) (6.25 μg/mL; 48 h) significantly upregulates the gene expression of type I collagen, type III collagen and KGF in the human fibroblast cell line Hs-68^[2].
TP4 (Nile tilapia piscidin) (0.3125-40 μg/mL; 48 h) promotes the proliferation of human keratinocyte HaCaT cells at concentrations as low as 10 μg/mL, and does not reduce cell viability within this concentration range^[2].
TP4 (Nile tilapia piscidin) (6.25 μg/mL; 48 h) significantly upregulates the expression of keratin 10 gene in human keratinocyte HaCaT cells, but has no effect on the expression of keratin 17^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	Hs-68
Concentration:	0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 μg/mL
Incubation Time:	48 h
Result:	Did not reduce Hs-68 cell viability at 0.3125, 0.625, 1.25, 2.5, 5, 10 μg/mL. Decreased Hs-68 cell viability at 20 and 40 μg/mL. Significantly increased Hs-68 cell proliferation at 2.5, 5, 10 μg/mL compared to the untreated group.

Real Time qPCR^[2]

Cell Line:	Hs-68
Concentration:	6.25 μg/mL
Incubation Time:	48 h
Result:	Significantly increased collagen I mRNA expression to a relative level of 2.5 compared to untreated cells (P < 0.001). Significantly increased collagen III mRNA expression to a relative level of 2.1 compared to untreated cells (P < 0.05). Significantly increased KGF mRNA expression to a relative level of 1.9 compared to untreated cells (P < 0.05).

Cell Proliferation Assay^[2]

Cell Line:	HaCaT
Concentration:	0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 μg/mL
Incubation Time:	48 h
Result:	Did not reduce HaCaT cell viability at 0.3125, 0.625, 1.25, 2.5, 5, 10 μg/mL. Decreased HaCaT cell viability at 20 and 40 μg/mL. Stimulated HaCaT cell proliferation at concentrations down to 10 μg/mL.

Real Time qPCR^[2]

Cell Line:	HaCaT
Concentration:	6.25 μg/mL
Incubation Time:	48 h
Result:	Significantly increased keratin 10 mRNA expression to a relative level of 1.5 compared to untreated cells (P < 0.05). Did not affect keratin 17 mRNA expression.

In Vivo

TP4 (2 mg/mouse; i.m.; single bolus injection) does not induce systemic toxic effects in healthy mice, with transient liver enzyme elevations resolving within 3 days^[2].
TP4 (0.005 mg/g; i.p.; single injection) provides time-dependent survival benefit in MRSA-septic mice, with 100% survival when administered 10 minutes post-infection, and effectively reduces bacterial burden in all tested organs^[2].
TP4 (50-100 mg/kg diet; p.o.; twice daily; 20 days) enhances innate immune responses, antioxidant activity, modulates cytokine gene expression, reduces tissue microbial load (in posterior kidney and intestine), and improves disease resistance against S. iniae in Nile tilapia, with the 100 mg/kg dose conferring the greatest reduction in cumulative mortality (to 30%)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Balb/c (female, 6-8 weeks old)^[2]
Dosage:	0.005 mg/g
Administration:	i.p.; single injection
Result:	Achieved 100% survival rate at 8 days post-infection when administered 10 minutes post-infection; achieved survival rates of 100%, 80%, 60%, and 50% when administered at 10, 60, 120, and 180 minutes post-infection, respectively. Reduced lethality to 0% at 48 hours post-treatment, eliminated bacterial burden in blood, and significantly reduced bacterial counts in peritoneum (1×10⁶ CFU/mL), spleen (3×10⁶ CFU/mL), liver (2.4×10⁶ CFU/mL), and mesenteric lymph nodes (6×10⁵ CFU/mL) compared to untreated infected controls.

Animal Model:	Balb/c (female, 6-8 weeks old)^[2]
Dosage:	50 µL of 2 mg/mL solution
Administration:	topical; single application to wound
Result:	Accelerated wound closure compared to vancomycin-treated wounds, with smaller wound size observed at 14 days post-infection and full closure by day 27. Reduced serum levels of proinflammatory cytokines TNF and IL-6 compared to MRSA-only infected mice at days 1, 2, and 3 post-infection, and significantly increased serum IL-1 levels compared to control wounds. Enhanced gene expression of EGF at day 3, TGF-β at day 7, and VEGF at day 14 post-infection compared to control and vancomycin-treated mice, with histological examination showing complete, architecturally normal epidermal regeneration.

Molecular Weight

2981.56

Formula

C₁₃₅H₂₂₆N₅₀O₂₇

CAS No.

1429184-62-2

Appearance

Solid

Color

White to off-white

Sequence

Phe-Ile-His-His-Ile-Ile-Gly-Gly-Leu-Phe-Ser-Ala-Gly-Lys-Ala-Ile-His-Arg-Leu-Ile-Arg-Arg-Arg-Arg-Arg

Sequence Shortening

FIHHIIGGLFSAGKAIHRLIRRRRR

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (33.54 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.3354 mL	1.6770 mL	3.3539 mL
5 mM	0.0671 mL	0.3354 mL	0.6708 mL
10 mM	0.0335 mL	0.1677 mL	0.3354 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (279 KB) SDS (254 KB)

COA (242 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Peng KC, et al. Five different piscidins from Nile tilapia, Oreochromis niloticus: analysis of their expressions and biological functions. PLoS One. 2012;7(11):e50263. [Content Brief]

[2]. Huang HN, et al. Tilapia Piscidin 4 (TP4) Stimulates Cell Proliferation and Wound Closure in MRSA-Infected Wounds in Mice. Mar Drugs. 2015;13(5):2813-2833. Published 2015 May 6. [Content Brief]

[3]. Zahran E, et al. Tilapia piscidin 4 (TP4) enhances immune response, antioxidant activity, intestinal health and protection against Streptococcus iniae infection in Nile tilapia[J]. Aquaculture, 2019, 513: 734451.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.3354 mL	1.6770 mL	3.3539 mL	8.3849 mL
	5 mM	0.0671 mL	0.3354 mL	0.6708 mL	1.6770 mL
	10 mM	0.0335 mL	0.1677 mL	0.3354 mL	0.8385 mL
	15 mM	0.0224 mL	0.1118 mL	0.2236 mL	0.5590 mL
	20 mM	0.0168 mL	0.0838 mL	0.1677 mL	0.4192 mL
	25 mM	0.0134 mL	0.0671 mL	0.1342 mL	0.3354 mL
	30 mM	0.0112 mL	0.0559 mL	0.1118 mL	0.2795 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.