TP4 (Nile tilapia piscidin)
Based on 1 Customer Validation
TP4 (Nile tilapia piscidin) is an orally active amphipathic α-helical cationic antimicrobial peptide. TP4 (Nile tilapia piscidin) exerts broad-spectrum bactericidal activity against Gram-positive and Gram-negative bacteria by disrupting cell membrane structures, and also exhibits certain hemolytic activity. TP4 (Nile tilapia piscidin) regulates the innate immune system and enhances the expression of lysozymes, cytokines and antioxidant enzymes to resist bacterial infection. TP4 (Nile tilapia piscidin) also stimulates the proliferation of keratinocytes and fibroblasts, and significantly promotes the healing of wounds infected with MRSA. TP4 (Nile tilapia piscidin) can be used in research related to bacterial infection, peritonitis, wound healing and other fields.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 1429184-62-2
- Formula: C135H226N50O27
- Molecular Weight:2981.56
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
TP4 (Nile tilapia piscidin) (10-30 μg/mL; 24 h) inhibits the proliferation of tilapia ovary (TO2) cells in vitro in a dose-dependent manner, and reduces the cell viability to approximately 35% at a concentration of 28 μg/mL[1].
TP4 (Nile tilapia piscidin) (0.3125-40 μg/mL; 48 h) promotes the proliferation of Hs-68 human fibroblasts at concentrations of 2.5, 5, and 10 μg/mL, and does not reduce cell viability at these concentrations[2].
TP4 (Nile tilapia piscidin) (6.25 μg/mL; 48 h) significantly upregulates the gene expression of type I collagen, type III collagen and KGF in the human fibroblast cell line Hs-68[2].
TP4 (Nile tilapia piscidin) (0.3125-40 μg/mL; 48 h) promotes the proliferation of human keratinocyte HaCaT cells at concentrations as low as 10 μg/mL, and does not reduce cell viability within this concentration range[2].
TP4 (Nile tilapia piscidin) (6.25 μg/mL; 48 h) significantly upregulates the expression of keratin 10 gene in human keratinocyte HaCaT cells, but has no effect on the expression of keratin 17[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hs-68
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Concentration:0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 μg/mL
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Incubation Time:48 h
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Result:Did not reduce Hs-68 cell viability at 0.3125, 0.625, 1.25, 2.5, 5, 10 μg/mL.
Decreased Hs-68 cell viability at 20 and 40 μg/mL.
Significantly increased Hs-68 cell proliferation at 2.5, 5, 10 μg/mL compared to the untreated group.
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Cell Line:Hs-68
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Concentration:6.25 μg/mL
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Incubation Time:48 h
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Result:Significantly increased collagen I mRNA expression to a relative level of 2.5 compared to untreated cells (P < 0.001).
Significantly increased collagen III mRNA expression to a relative level of 2.1 compared to untreated cells (P < 0.05).
Significantly increased KGF mRNA expression to a relative level of 1.9 compared to untreated cells (P < 0.05).
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Cell Line:HaCaT
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Concentration:0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 μg/mL
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Incubation Time:48 h
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Result:Did not reduce HaCaT cell viability at 0.3125, 0.625, 1.25, 2.5, 5, 10 μg/mL.
Decreased HaCaT cell viability at 20 and 40 μg/mL.
Stimulated HaCaT cell proliferation at concentrations down to 10 μg/mL.
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Cell Line:HaCaT
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Concentration:6.25 μg/mL
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Incubation Time:48 h
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Result:Significantly increased keratin 10 mRNA expression to a relative level of 1.5 compared to untreated cells (P < 0.05).
Did not affect keratin 17 mRNA expression.
TP4 (0.005 mg/g; i.p.; single injection) provides time-dependent survival benefit in MRSA-septic mice, with 100% survival when administered 10 minutes post-infection, and effectively reduces bacterial burden in all tested organs[2].
TP4 (50-100 mg/kg diet; p.o.; twice daily; 20 days) enhances innate immune responses, antioxidant activity, modulates cytokine gene expression, reduces tissue microbial load (in posterior kidney and intestine), and improves disease resistance against S. iniae in Nile tilapia, with the 100 mg/kg dose conferring the greatest reduction in cumulative mortality (to 30%)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Balb/c (female, 6-8 weeks old)[2]
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Dosage:0.005 mg/g
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Administration:i.p.; single injection
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Result:Achieved 100% survival rate at 8 days post-infection when administered 10 minutes post-infection; achieved survival rates of 100%, 80%, 60%, and 50% when administered at 10, 60, 120, and 180 minutes post-infection, respectively.
Reduced lethality to 0% at 48 hours post-treatment, eliminated bacterial burden in blood, and significantly reduced bacterial counts in peritoneum (1×106 CFU/mL), spleen (3×106 CFU/mL), liver (2.4×106 CFU/mL), and mesenteric lymph nodes (6×105 CFU/mL) compared to untreated infected controls.
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Animal Model:Balb/c (female, 6-8 weeks old)[2]
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Dosage:50 µL of 2 mg/mL solution
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Administration:topical; single application to wound
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Result:Accelerated wound closure compared to vancomycin-treated wounds, with smaller wound size observed at 14 days post-infection and full closure by day 27.
Reduced serum levels of proinflammatory cytokines TNF and IL-6 compared to MRSA-only infected mice at days 1, 2, and 3 post-infection, and significantly increased serum IL-1 levels compared to control wounds.
Enhanced gene expression of EGF at day 3, TGF-β at day 7, and VEGF at day 14 post-infection compared to control and vancomycin-treated mice, with histological examination showing complete, architecturally normal epidermal regeneration.
Chemical Information
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CAS No. 1429184-62-2
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Appearance Solid
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Molecular Weight 2981.56
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Formula C135H226N50O27
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Color White to off-white
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Sequence
Phe-Ile-His-His-Ile-Ile-Gly-Gly-Leu-Phe-Ser-Ala-Gly-Lys-Ala-Ile-His-Arg-Leu-Ile-Arg-Arg-Arg-Arg-Arg
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Sequence Shortening
FIHHIIGGLFSAGKAIHRLIRRRRR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 100 mg/mL (33.54 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Peng KC, et al. Five different piscidins from Nile tilapia, Oreochromis niloticus: analysis of their expressions and biological functions. PLoS One. 2012;7(11):e50263. [Content Brief]
[2]. Huang HN, et al. Tilapia Piscidin 4 (TP4) Stimulates Cell Proliferation and Wound Closure in MRSA-Infected Wounds in Mice. Mar Drugs. 2015;13(5):2813-2833. Published 2015 May 6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.3354 mL | 1.6770 mL | 3.3539 mL | 8.3849 mL |
| 5 mM | 0.0671 mL | 0.3354 mL | 0.6708 mL | 1.6770 mL | |
| 10 mM | 0.0335 mL | 0.1677 mL | 0.3354 mL | 0.8385 mL | |
| 15 mM | 0.0224 mL | 0.1118 mL | 0.2236 mL | 0.5590 mL | |
| 20 mM | 0.0168 mL | 0.0838 mL | 0.1677 mL | 0.4192 mL | |
| 25 mM | 0.0134 mL | 0.0671 mL | 0.1342 mL | 0.3354 mL | |
| 30 mM | 0.0112 mL | 0.0559 mL | 0.1118 mL | 0.2795 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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