Cat. No.: HY-151395

Tubulin polymerization-IN-34

Tubulin polymerization-IN-34 is an inhibitor of [1,2]oxazoloisoindoles tubulin polymerization, demonstrates high selectivity against marginal zone lymphoma VL51 cell line.

For research use only. We do not sell to patients.

CAS No.: 3051788-87-2
Formula: C₃₁H₃₅N₃O₆
Molecular Weight:545.63

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Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

Tubulin polymerization-IN-34 Chemical Structure

CAS No.: 3051788-87-2

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

Tubulin polymerization-IN-34 is an inhibitor of [1,2]oxazoloisoindoles tubulin polymerization, demonstrates high selectivity against marginal zone lymphoma VL51 cell line^[1].

IC₅₀ & Target

[1,2]oxazoloisoindoles tubulin polymerization^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	GI₅₀	0.77 μM Compound: 17i	Antiproliferative activity against human 786-0 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human 786-0 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
A498	GI₅₀	0.32 μM Compound: 17i	Antiproliferative activity against human A498 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human A498 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
A549	GI₅₀	1.13 μM Compound: 17i	Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
ACHN	GI₅₀	0.63 μM Compound: 17i	Antiproliferative activity against human ACHN cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human ACHN cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
BT-549	GI₅₀	1.34 μM Compound: 17i	Antiproliferative activity against human BT-549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human BT-549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
CAKI-1	GI₅₀	0.38 μM Compound: 17i	Antiproliferative activity against human CAKI-1 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human CAKI-1 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
CCRF-CEM	GI₅₀	1.26 μM Compound: 17i	Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
COLO 205	GI₅₀	0.59 μM Compound: 17i	Antiproliferative activity against human COLO 205 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human COLO 205 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
DU-145	GI₅₀	1.99 μM Compound: 17i	Antiproliferative activity against human DU-145 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human DU-145 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
EKVX	GI₅₀	1.4 μM Compound: 17i	Antiproliferative activity against human EKVX cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human EKVX cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HBL1	IC₅₀	0.5 μM Compound: 17i	Antiproliferative activity against human HBL1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Antiproliferative activity against human HBL1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 36242921]
HCC 2998	GI₅₀	2.17 μM Compound: 17i	Antiproliferative activity against human HCC 2998 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HCC 2998 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HCT-116	GI₅₀	0.5 μM Compound: 17i	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HCT-15	GI₅₀	0.51 μM Compound: 17i	Antiproliferative activity against human HCT-15 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-15 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HL-60(TB)	GI₅₀	0.39 μM Compound: 17i	Antiproliferative activity against human HL-60(TB) cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HL-60(TB) cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HOP-62	GI₅₀	1 μM Compound: 17i	Antiproliferative activity against human HOP-62 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HOP-62 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HOP-92	GI₅₀	0.9 μM Compound: 17i	Antiproliferative activity against human HOP-92 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HOP-92 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
Hs-578T	GI₅₀	0.5 μM Compound: 17i	Antiproliferative activity against human Hs-578T cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human Hs-578T cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
HT-29	GI₅₀	0.64 μM Compound: 17i	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
IGROV-1	GI₅₀	0.81 μM Compound: 17i	Antiproliferative activity against human IGROV-1 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human IGROV-1 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
K562	GI₅₀	0.43 μM Compound: 17i	Antiproliferative activity against human K562 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
KM12	GI₅₀	0.4 μM Compound: 17i	Antiproliferative activity against human KM12 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human KM12 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
LOX IMVI	GI₅₀	0.62 μM Compound: 17i	Antiproliferative activity against human LOX IMVI cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human LOX IMVI cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
M14	GI₅₀	0.56 μM Compound: 17i	Antiproliferative activity against human M14 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human M14 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
Malme-3M	GI₅₀	0.5 μM Compound: 17i	Antiproliferative activity against human Malme-3M cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human Malme-3M cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
MCF7	GI₅₀	0.36 μM Compound: 17i	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
MCF7	IC₅₀	0.47 μM Compound: 17i	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition Cytotoxicity against human MCF7 cells assessed as cell growth inhibition	[PMID: 36242921]
MDA-MB-231	GI₅₀	2.25 μM Compound: 17i	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
MDA-MB-435	GI₅₀	0.25 μM Compound: 17i	Antiproliferative activity against human MDA-MB-435 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-435 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
MDA-MB-468	GI₅₀	0.34 μM Compound: 17i	Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
MOLT-4	GI₅₀	3.06 μM Compound: 17i	Antiproliferative activity against human MOLT-4 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MOLT-4 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
NCI/ADR-RES	GI₅₀	0.43 μM Compound: 17i	Antiproliferative activity against human NCI-ADR-RES cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-ADR-RES cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
NCI-H226	GI₅₀	0.89 μM Compound: 17i	Antiproliferative activity against human NCI-H226 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H226 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
NCI-H23	GI₅₀	2.17 μM Compound: 17i	Antiproliferative activity against human NCI-H23 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H23 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
NCI-H460	GI₅₀	0.52 μM Compound: 17i	Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
NCI-H522	GI₅₀	0.3 μM Compound: 17i	Antiproliferative activity against human NCI-H522 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H522 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
OVCAR-4	GI₅₀	6.84 μM Compound: 17i	Antiproliferative activity against human OVCAR-4 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human OVCAR-4 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
OVCAR-8	GI₅₀	1.92 μM Compound: 17i	Antiproliferative activity against human OVCAR-8 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human OVCAR-8 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
PC-3	GI₅₀	0.65 μM Compound: 17i	Antiproliferative activity against human PC-3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human PC-3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
RPMI-8226	GI₅₀	0.77 μM Compound: 17i	Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
RXF 393	GI₅₀	0.29 μM Compound: 17i	Antiproliferative activity against human RXF 393 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human RXF 393 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SF-268	GI₅₀	0.78 μM Compound: 17i	Antiproliferative activity against human SF-268 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SF-268 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SF-295	GI₅₀	0.47 μM Compound: 17i	Antiproliferative activity against human SF-295 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SF-295 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SF-539	GI₅₀	0.44 μM Compound: 17i	Antiproliferative activity against human SF-539 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SF-539 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SK-MEL-2	GI₅₀	0.55 μM Compound: 17i	Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SK-MEL-28	GI₅₀	>100 μM Compound: 17i	Antiproliferative activity against human SK-MEL-28 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SK-MEL-28 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SK-MEL-5	GI₅₀	1.86 μM Compound: 17i	Antiproliferative activity against human SK-MEL-5 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SK-MEL-5 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SK-OV-3	GI₅₀	0.97 μM Compound: 17i	Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SN12C	GI₅₀	3.81 μM Compound: 17i	Antiproliferative activity against human SN12C cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SN12C cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SNB-19	GI₅₀	0.86 μM Compound: 17i	Antiproliferative activity against human SNB-19 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SNB-19 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SNB-75	GI₅₀	0.31 μM Compound: 17i	Antiproliferative activity against human SNB-75 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SNB-75 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SR	GI₅₀	0.38 μM Compound: 17i	Antiproliferative activity against human SR cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SR cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
SU-DHL10	IC₅₀	0.5 μM Compound: 17i	Antiproliferative activity against human SU-DHL-10 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Antiproliferative activity against human SU-DHL-10 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 36242921]
SW-620	GI₅₀	0.43 μM Compound: 17i	Antiproliferative activity against human SW620 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SW620 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
T47D	GI₅₀	>100 μM Compound: 17i	Antiproliferative activity against human T47D cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human T47D cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
TK-10	GI₅₀	>100 μM Compound: 17i	Antiproliferative activity against human TK-10 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human TK-10 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
U-251	GI₅₀	0.88 μM Compound: 17i	Antiproliferative activity against human U-251 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human U-251 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
UACC-257	GI₅₀	1 μM Compound: 17i	Antiproliferative activity against human UACC-257 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human UACC-257 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
UACC-62	GI₅₀	0.61 μM Compound: 17i	Antiproliferative activity against human UACC-62 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human UACC-62 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]
UO-31	GI₅₀	0.76 μM Compound: 17i	Antiproliferative activity against human UO-31 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human UO-31 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36242921]

In Vitro

Tubulin polymerization-IN-34 (compound 17i) (10 nM-100 μM; 72 h) has antiproliferative activity against 9 NCI subpanels (leukemia, non-small-cell lung, colon, central nervous system, melanoma, ovarian, renal, prostate, breast) with GI₅₀s ranging from 0.25 μM to 6.84 μM, and a mean graph_mid point (MG_MID) values of 0.95 μM^[1].
Tubulin polymerization-IN-34 (0.15-10 μM; 72 h) shows potent growth inhibitory effects on different lymphoma lines, and demonstrates high selectivity against the VL51 cell line (Marginal zone lymphoma, IC₅₀=0.2 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Marginal zone lymphoma, mantle cell lymphoma, activated B-cell like diffuse large B cell lymphoma, germinal center B-cell-like diffuse large B cell lymphoma cells
Concentration:

0.15-10 μM
Incubation Time:

72 h
Result:

Inhibited different lymphomas, with IC₅₀s of 0.2 μM (Marginal zone lymphoma); 0.5 μM (mantle cell lymphoma); 0.5 μM (activated B-cell like diffuse large B cell lymphoma); 0.5 μM (germinal center B-cell-like diffuse large B cell lymphoma), respectively.

Chemical Information

CAS No. 3051788-87-2
Molecular Weight 545.63
Formula C₃₁H₃₅N₃O₆
SMILES

COC1=C(OC)C(OC)=CC(C2=C3CCC4=C(ON=C4CN5CCOCC5)C3=CN2CC6=CC=C(OC)C=C6)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Marilia Barreca, et al. Development of [1,2]oxazoloisoindoles tubulin polymerization inhibitors: Further chemical modifications and potential therapeutic effects against lymphomas, European Journal of Medicinal Chemistry. 2022, 114744, ISSN 0223-5234.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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