2. Metabolic Enzyme/Protease Immunology/Inflammation
  3. Tyrosinase MHC
  4. Tyrosinase (192-200), human mouse

Tyrosinase (192-200), human mouse is a HLA-B*4403-restricted tyrosinase epitope with affinity for HLA-B4403 and HLA-B4402. Tyrosinase (192-200), human mouse binds to HLA-B*4403 to form a complex, which is recognized by the autologous cytotoxic T lymphocyte clone 22/31 derived from melanoma. Tyrosinase (192-200), human mouse binds to HLA-B4402 to form a complex, which is recognized by the CTL clone 329B/5 derived from healthy HLA-B4402 donors. Tyrosinase (192-200), human mouse is applicable to melanoma-related research.

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Tyrosinase (192-200), human mouse

Tyrosinase (192-200), human mouse Chemical Structure

CAS No. : 170294-35-6

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Tyrosinase (192-200), human mouse Related Antibodies

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Description

Tyrosinase (192-200), human mouse is a HLA-B*4403-restricted tyrosinase epitope with affinity for HLA-B4403 and HLA-B4402. Tyrosinase (192-200), human mouse binds to HLA-B*4403 to form a complex, which is recognized by the autologous cytotoxic T lymphocyte clone 22/31 derived from melanoma. Tyrosinase (192-200), human mouse binds to HLA-B4402 to form a complex, which is recognized by the CTL clone 329B/5 derived from healthy HLA-B4402 donors. Tyrosinase (192-200), human mouse is applicable to melanoma-related research[1][2].

In Vitro

Tyrosinase (192-200), human mouse (0.1-1000 nM; 30 min pre-incubation with target cells, 4 h CTL incubation) potently sensitizes HLA-B*4403-positive LG2-EBV lymphoblastoid cells to lysis by autologous CTL clone 22/31, with maximal lysis observed at concentrations of 100 nM and above[1].
Tyrosinase (192-200), human mouse (10-1000 nM; 30 min pre-incubation with target cells, 4 h CTL incubation) is recognized by CTL clone 22/31 in the context of HLA-B4403 and by CTL clone 329B/5 in the context of HLA-B4402, inducing dose-dependent lysis of the respective HLA subtype-positive lymphoblastoid cell lines[1].
Tyrosinase (192-200), human mouse (1-50 μM; 4 h pre-incubation with adherent cells, re-stimulation on days 7, 14, and weekly thereafter) effectively stimulates CD8+ T lymphocytes from a healthy HLA-B4402 donor to generate CTL clone 329B/5, which recognizes the peptide presented by HLA-B4402[1].
Tyrosinase (192-200), human mouse-specific HLA-B4403-restricted CTL clone efficiently lyses tyrosinase-expressing HLA-B4403+ cells, with half-maximal lysis of peptide-pulsed targets occurring at a peptide concentration of 1 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tyrosinase (192-200), human mouse Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: EBV-transformed lymphoblastoid cell line LG2-EBV (HLA-B*4403 positive), autologous CTL clone 22/31
Concentration: 0.1, 1, 10, 100, 1000 nM (30 min pre-incubation with target cells)
Incubation Time: 4 h (CTL incubation)
Result: Sensitized LG2-EBV cells to lysis by CTL clone 22/31 in a dose-dependent manner.
Achieved maximal specific lysis (≥95%) at 100 nM and 1000 nM concentrations.
Reduced maximal specific lysis to only ~10% at 1000 nM when the N-terminal serine residue was removed (forming peptide EIWRDIDFA).

Cell Cytotoxicity Assay[1]

Cell Line: EBV-transformed lymphoblastoid cell lines expressing HLA-B*4403 (MZ2-EBV, LB17-EBV, LG2-EBV) or HLA-B*4402 (SK29-EBV, BI2-EBV, LB33-EBV), CTL clone 22/31, CTL clone 329B/5
Concentration: 10, 100, 1000 nM (30 min pre-incubation with target cells)
Incubation Time: 4 h (CTL incubation)
Result: Induced CTL clone 22/31 to potently lyse all HLA-B*4403-positive lymphoblastoid cell lines, with maximal specific lysis (≥70%) achieved at 1000 nM.
Caused minimal to no lysis of HLA-B*4402-positive cell lines by CTL clone 22/31.
Induced CTL clone 329B/5 to potently lyse HLA-B*4402-positive LB33-EBV cells, with maximal specific lysis ≥80% at 1000 nM.
Resulted in minimal lysis of HLA-B*4403-positive cell lines by CTL clone 329B/5.
Molecular Weight

1180.27

Formula

C54H77N13O17
CAS No.
Sequence Shortening

SEIWRDIDF
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Tyrosinase (192-200), human mouse Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tyrosinase (192-200), human mouse170294-35-6TyrosinaseMHCMajor Histocompatibility ComplexTyrosinase (192-200)human mouseHLA-B*4402CD8+ T lymphocytesCOS-7 cellsSK29-MELLG2-EBV lymphoblastoid cellsHLA-B*4403cytolytic T lymphocytes clone 22/31melanomaCTL clone 329B/5Inhibitorinhibitorinhibit

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