Tyrosinase (192-200), human mouse

Tyrosinase (192-200), human mouse (0.1-1000 nM; 30 min pre-incubation with target cells, 4 h CTL incubation) potently sensitizes HLA-B*4403-positive LG2-EBV lymphoblastoid cells to lysis by autologous CTL clone 22/31, with maximal lysis observed at concentrations of 100 nM and above^[1].
Tyrosinase (192-200), human mouse (10-1000 nM; 30 min pre-incubation with target cells, 4 h CTL incubation) is recognized by CTL clone 22/31 in the context of HLA-B4403 and by CTL clone 329B/5 in the context of HLA-B4402, inducing dose-dependent lysis of the respective HLA subtype-positive lymphoblastoid cell lines^[1].
Tyrosinase (192-200), human mouse (1-50 μM; 4 h pre-incubation with adherent cells, re-stimulation on days 7, 14, and weekly thereafter) effectively stimulates CD8⁺ T lymphocytes from a healthy HLA-B4402 donor to generate CTL clone 329B/5, which recognizes the peptide presented by HLA-B4402^[1].
Tyrosinase (192-200), human mouse-specific HLA-B4403-restricted CTL clone efficiently lyses tyrosinase-expressing HLA-B4403⁺ cells, with half-maximal lysis of peptide-pulsed targets occurring at a peptide concentration of 1 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.