Cat. No.: HY-N0772

Isomangiferin

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Isomangiferin is an orally active xanthone C-glucoside, and its chemical structure is similar to Mangiferin (HY-N0290). Isomangiferin is an effective VEGFR-2 kinase inhibitor, which can induces cell apoptosis, inhibit the growth, metastasis and angiogenesis of breast cancer. Isomangiferin exerts anti-inflammatory effects by inhibiting the HMGB1/NLRP3/NF-κB signaling pathway, thereby improving the renal function indicators of diabetic mice. Isomangiferin exhibits inhibitory effects on various bacteria and herpes simplex virus type 1 (HSV-1). Isomangiferin promotes the migration and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and reduces cell apoptosis and the production of ROS by activating the AMPK/ACC pathway, thereby facilitating fracture healing.

For research use only. We do not sell to patients.

Purity: 99.60%
CAS No.: 24699-16-9
Formula: C₁₉H₁₈O₁₁
Molecular Weight:422.34

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isomangiferin Chemical Structure

CAS No.: 24699-16-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All VEGFR Isoforms :

All NOD-like Receptor (NLR) Isoforms :

All NF-κB Isoforms :

All AMPK Isoforms :

All HSV Isoforms :

In Vitro

Isomangiferin inhibits HSV-1 that exceeds Acyclovir (HY-17422), Idoxuridine (HY-B0307), and Cyclocytidine (HY-N0093) in log by 0.27-0.50 in log determination and exhibits the average plaque reduction rate of 69.5%^[1].
Isomangiferin shows better antibacterial potential than Mangiferin against all the tested strain including B. subtilis, S. aureus, K. pneumoniae, S. Setubal, E. coli with MICs ≤ 250 μg/mL^[2].
Isomangiferin shows poor cytotoxicity towards Brine shrimp nauplii with a LD₅₀ of 768.92 mg/mL^[2].
Isomangiferin (2.5-10 μM, 24 h) promotes osteogenic differentiation and migration of H₂O₂-treated bone marrow mesenchymal stem cells (BMSCs), reduces apoptosis and reactive oxygen species production, and activates the AMP-activated protein kinase/acetyl-CoA carboxylase (AMPK/ACC) pathway^[3].
Isomangiferin (1 μM, 48 h) decreases the viability of breast cancer cells (MDA-MB-231, T47D, MCF7, SKBR3, 4T1) with IC₅₀s of approximately 1 μM, , while the IC₅₀ for the MCF-10A cells exceeded 100 μM^[5].
Isomangiferin (1 μM, 5 days) inhibits microvessel sprouting and vascular tubulogenesis of endothelial cells^[5].
Isomangiferin (0-5 μM, 1-36 h) inhibits migration and invasion and induces breast cancer cell apoptosis and impairs cell adhesion in MDA-MB-231 cells through VEGFR-2-mediated signaling pathway^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

BMSCs stimulated with H₂O₂
Concentration:

2.5, 5 and 10 μM
Incubation Time:

24 h
Result:

Dose-dependently restored cell viability.

Cell Migration Assay ^[3]

Cell Line:

BMSCs stimulated with H₂O₂
Concentration:

2.5, 5 and 10 μM
Incubation Time:

24 h
Result:

Restored the migration ability by 2.1 times at 10 μM.

Apoptosis Analysis^[3]

Cell Line:

BMSCs stimulated with H₂O₂
Concentration:

2.5, 5 and 10 μM
Incubation Time:

24 h
Result:

Reduced the apoptosis rate to 11.3% at 10 μM.

ELISA Assay^[3]

Cell Line:

BMSCs stimulated with H₂O₂
Concentration:

2.5, 5 and 10 μM
Incubation Time:

24 h
Result:

Restored the ALP activity to 90% of its normal level at 10 μM.
Reduced the level of ROS by 60% at 10 μM.

Western Blot Analysis^[3]

Cell Line:

BMSCs stimulated with H₂O₂
Concentration:

2.5, 5 and 10 μM
Incubation Time:

24 h
Result:

Induced the upregulation of Runx2 and BMP2 expression.
Downregulated the pro-apoptotic protein Bax, upregulated the anti-apoptotic protein Bcl-2 and inhibited the activation of caspase-3.
Significantly increase the levels of p-AMPK and p-ACC (Ser79).

Cell Migration Assay ^[5]

Cell Line:

MDA-MB-231 cells
Concentration:

0, 0.5, 1, 2.5 and 5 μM
Incubation Time:

6 h
Result:

Showed dose-dependent inhibition, and almost completely inhibited migration at 5 μM.

Cell Invasion Assay^[5]

Cell Line:

MDA-MB-231 cells
Concentration:

0, 0.5, 1, 2.5 and 5 μM
Incubation Time:

8 to 10 h
Result:

Showed dose-dependent inhibition, and almost completely inhibited invasion at 5 μM.

Apoptosis Analysis^[5]

Cell Line:

MDA-MB-231 cells
Concentration:

0, 0.5, 1, 2.5 and 5 μM
Incubation Time:

36 h
Result:

Increased the apoptosis rate from 7.15% to 13.06% at a concentration of 5 μM.

Western Blot Analysis^[5]

Cell Line:

MDA-MB-231 cells
Concentration:

0, 0.5, 1, 2.5 μM
Incubation Time:

24 h
Result:

Activated caspase-3 and PARP cleavage.

In Vivo

Isomangiferin (10-20 mg/kg, p.o., once daily for 12 weeks) provides protection against renal injury via inhibiting HMGB1/NLRP3/ NF-κB signaling in a diabetic mouse model^[4].
Isomangiferin (10 mg/kg, i.p., once daily for a month) inhibits breast cancer growth and blocks the VEGFR-2 pathway in the xenograft model^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Type 2 diabetes mellitus (T2DM) model established in mice (C57BL/KsJ db/db mice, male) and male db/m mice^[4]
Dosage:

10 and 20 mg/kg
Administration:

Oral administration (p.o.), once daily for 12 weeks
Result:

Reduced UA and Cr levels in Db/Db mice.
Reduced serum insulin levels and improved lipid profiles in Db/Db mice.
Attenuated kidney damage.
Reduced inflammatory cytokine levels in serum and the kidney.
Inhibited HMGB1/NACHT leucine-rich repeat- and PYD-containing 3 (NLRP3)/NF-κB pathway in Db/Db mice.

Animal Model:

MDA-MB-231 induced xenograft model established in five-week-old male BALB/c nude mice^[5]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p.), once daily for a month
Result:

Significantly reduced the volume and weight of the tumor.
Significantly reduced the expression of p-VEGFR2 and CD31. Reduced the microvessel density (MVD) within the tumor tissue and the number of p-VEGFR2 positive cells.

Chemical Information

CAS No. 24699-16-9
Appearance Solid
Molecular Weight 422.34
Formula C₁₉H₁₈O₁₁
Color Light yellow to green yellow
SMILES

OC1=CC(O)=C2C(OC3=CC(O)=C(O)C=C3C2=O)=C1[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
Structure Classification
- Phenols
- Polyphenols
- Phenols
- Xanthones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (4)

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Journal Impact Factor

Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
University of Paris

2022 Sep 19.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (118.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3678 mL

11.8388 mL

23.6776 mL

5 mM

0.4736 mL

2.3678 mL

4.7355 mL

10 mM

0.2368 mL

1.1839 mL

2.3678 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration Concentration

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (292 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zheng MS, et al. Antiviral effect of mangiferin and isomangiferin on herpes simplex virus. Chin Med J (Engl). 1990 Feb;103(2):160-5. [Content Brief]

[2]. Ishaque M, et al. Xanthone C-glycosides isomers purified from Dryopteris ramosa (Hope) C. Chr. with bactericidal and cytotoxic prospects. Saudi J Biol Sci. 2022 Feb;29(2):1191-1196. [Content Brief]

[3]. Gao B, et al. Isomangiferin promotes the migration and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Open Life Sci. 2024 Jul 20;19(1):20220884. doi: 10.1515/biol-2022-0884. PMID: 39035458; PMCID: PMC11259999. [Content Brief]

[4]. Yue S, et al. Isomangiferin Attenuates Renal Injury in Diabetic Mice via Inhibiting Inflammation. Diabetes Metab Syndr Obes. 2020 Nov 10;13:4273-4280. [Content Brief]

[5]. Wang B, et al. Isomangiferin, a Novel Potent Vascular Endothelial Growth Factor Receptor 2 Kinase Inhibitor, Suppresses Breast Cancer Growth, Metastasis and Angiogenesis. J Breast Cancer. 2018 Mar;21(1):11-20. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3678 mL	11.8388 mL	23.6776 mL	59.1940 mL
	5 mM	0.4736 mL	2.3678 mL	4.7355 mL	11.8388 mL
	10 mM	0.2368 mL	1.1839 mL	2.3678 mL	5.9194 mL
	15 mM	0.1579 mL	0.7893 mL	1.5785 mL	3.9463 mL
	20 mM	0.1184 mL	0.5919 mL	1.1839 mL	2.9597 mL
	25 mM	0.0947 mL	0.4736 mL	0.9471 mL	2.3678 mL
	30 mM	0.0789 mL	0.3946 mL	0.7893 mL	1.9731 mL
	40 mM	0.0592 mL	0.2960 mL	0.5919 mL	1.4799 mL
	50 mM	0.0474 mL	0.2368 mL	0.4736 mL	1.1839 mL
	60 mM	0.0395 mL	0.1973 mL	0.3946 mL	0.9866 mL
	80 mM	0.0296 mL	0.1480 mL	0.2960 mL	0.7399 mL
	100 mM	0.0237 mL	0.1184 mL	0.2368 mL	0.5919 mL

Isomangiferin

4 Cited Publications

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Isomangiferin

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