2. Anti-infection Apoptosis
  3. HSV Apoptosis Antibiotic Bacterial
  4. Acyclovir

Acyclovir  (Synonyms: Aciclovir; Acycloguanosine)

Cat. No.: HY-17422 Purity: 98.45%
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Acyclovir (Aciclovir) is a potent, orally active antiviral agent. Acyclovir has antiherpetic activity with IC50 values of 0.85 μM and 0.86 μM for HSV-1 and HSV-2, respectively. Acyclovir induces cell cycle perturbation and apoptosis. Acyclovir prevents bacterial infections during induction therapy for acute leukaemia.

For research use only. We do not sell to patients.

Acyclovir Chemical Structure

Acyclovir Chemical Structure

CAS No. : 59277-89-3

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10 mM * 1 mL in DMSO
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Based on 8 publication(s) in Google Scholar

Other Forms of Acyclovir:

Acyclovir Related Antibodies

Top Publications Citing Use of Products

    Acyclovir purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Mar 27;162:114595.  [Abstract]

    Acyclovir (ACV; 2.5 μM; 48 h) significantly inhibits the expression of gD in HSV-2-infected HeLa, Vero, Beas-2B and HaCaT cells.

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    Description

    Acyclovir (Aciclovir) is a potent, orally active antiviral agent. Acyclovir has antiherpetic activity with IC50 values of 0.85 μM and 0.86 μM for HSV-1 and HSV-2, respectively. Acyclovir induces cell cycle perturbation and apoptosis. Acyclovir prevents bacterial infections during induction therapy for acute leukaemia[1][2][3][4].

    IC50 & Target[2]

    HSV-1

    0.85 μM (IC50)

    HSV-2

    0.86 μM (IC50)

    In Vitro

    Acyclovir (Aciclovir, 3-100 μM; 24-72 hours; Jurkat, U937, and K562 leukemia cells) reduces cell viability in a dose- and time-dependent[1].
    ? Acyclovir (Aciclovir, 10-100 μM; 24-72 hours; Jurkat cells) blocks DNA synthesis, thereby arresting the cell cycle in G2/M and S phases and increasing the sub-G1 hypodiploid peak in a dose-dependent manner[1].
    ? Acyclovir (Aciclovir, 10-100 μM; 24-72 hours; Jurkat cells) induces apoptosis through activates caspase-3 and presences nuclear DNA fragmentation[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acyclovir Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Jurkat, U937 and K562 leukemia cells
    Concentration: 3, 10, 30 and 100 µM
    Incubation Time: 24, 48 and 72 hours
    Result: Showed a dose- and time-dependent reduction of cell viability.

    Apoptosis Analysis[3]

    Cell Line: Jurkat cells
    Concentration: 10 and 100 µM
    Incubation Time: 24, 48 and 72 hours
    Result: Increased of caspase-3 activity and cleavaged the internucleosomal DNA.

    Cell Cycle Analysis[1]

    Cell Line: Jurkat cells
    Concentration: 10 and 100 µM
    Incubation Time: 24, 48 and 72 hours
    Result: Revealed a dose-dependent accumulation of cells in S phase after 24 and 48 h. Showed a dose-dependent increase of the sub-G1 hypodiploid peak after 72 h.
    In Vivo

    Acyclovir (20 mg/kg; p.o.; three times daily; for 10 days; BALB/c mice) treatment in infected mice suppresses the development of skin lesions and results in a dissociation between DTH response and antibody production[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Specific-pathogen-free BALB/c mice (7-week-old) infected with HSV-1[1]
    Dosage: 20 mg/kg
    Administration: Oral administration; three times daily; for 10 days
    Result: Suppressed the development of skin lesions and resulted in a dissociation between DTH response and antibody production.
    Clinical Trial
    Molecular Weight

    225.20

    Formula

    C8H11N5O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1NC(N)=NC2=C1N=CN2COCCO
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (222.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.4405 mL 22.2025 mL 44.4050 mL
    5 mM 0.8881 mL 4.4405 mL 8.8810 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 20 mg/mL (88.81 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% CMC/saline water

      Solubility: 20 mg/mL (88.81 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.34%

    COA (248 KB) RP-HPLC (126 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.4405 mL 22.2025 mL 44.4050 mL 111.0124 mL
    5 mM 0.8881 mL 4.4405 mL 8.8810 mL 22.2025 mL
    10 mM 0.4440 mL 2.2202 mL 4.4405 mL 11.1012 mL
    15 mM 0.2960 mL 1.4802 mL 2.9603 mL 7.4008 mL
    20 mM 0.2220 mL 1.1101 mL 2.2202 mL 5.5506 mL
    25 mM 0.1776 mL 0.8881 mL 1.7762 mL 4.4405 mL
    30 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7004 mL
    40 mM 0.1110 mL 0.5551 mL 1.1101 mL 2.7753 mL
    50 mM 0.0888 mL 0.4440 mL 0.8881 mL 2.2202 mL
    60 mM 0.0740 mL 0.3700 mL 0.7401 mL 1.8502 mL
    80 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL
    100 mM 0.0444 mL 0.2220 mL 0.4440 mL 1.1101 mL
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    Acyclovir Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acyclovir59277-89-3Aciclovir AcycloguanosineHSVApoptosisAntibioticBacterialHerpes simplex virusimmunosuppressionantibodythymidinekinaseDNApolymerasetriphosphateanti-herpeticInhibitorinhibitorinhibit

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