1-Pyrenebutyric acid
Based on 1 Customer Validation
1-Pyrenebutyric acid is a pyrene-based fluorescent probe compound. The fluorescence lifetime of 1-Pyrenebutyric acid is quenched by molecular oxygen and intracellular ROS, thus it serves as a fluorescent probe for detecting ROS and oxygen concentration through fluorescence lifetime measurements. 1-Pyrenebutyric acid undergoes electrostatic interactions with arginine-rich peptides, increasing their net hydrophobicity, promoting their direct translocation across lipid bilayers, and enhancing cytosolic delivery. 1-Pyrenebutyric acid is used for intracellular reactive oxygen species detection and research related to arginine-rich peptide-mediated cytosolic delivery.
For research use only. We do not sell to patients.
- Purity: 97.61%
- CAS No.: 3443-45-6
- Formula: C20H16O2
- Molecular Weight:288.35
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
1-Pyrenebutyric acid (pyrenebutyrate) (50 μM; 2 min) enables rapid, non-membrane-disrupting direct cytosolic and nuclear delivery of 10 μM R8-Alexa peptide in HeLa cells, efficiently delivers 10 μM R8-EGFP protein to the cytosol, and accelerates the internalization of 5 μM R8-PAD peptide, thereby triggering significant mitochondrial depolarization[1].
1-Pyrenebutyric acid (50 μM; 10 min) enhances the cell-killing effect of 5 μM R8-PAD in HeLa cells, reducing cell viability to approximately 20%[1].
1-Pyrenebutyric acid (50 μM; 2 min) rapidly distributes to the perinuclear membrane compartment of HeLa cells and is immediately removed by PBS washing[1].
1-Pyrenebutyric acid (PBA) (0.6 μM; 20 min) exhibits oxygen- and reactive oxygen species-dependent fluorescence lifetime quenching in CCRF-CEM human lymphoblasts, and its fluorescence lifetime signal is independent of the intracellular probe accumulation level[2].
1-Pyrenebutyric acid (0.6 μM; 20 min) detects an 80% increase in reactive oxygen species in CCRF-CEM cells after treatment with Doxorubicin (HY-15142A) (1.7 μM; 3 days), and also detects a 67% decrease in intracellular reactive oxygen species after treatment with cysteamine (1 mM; 3 days)[2].
1-Pyrenebutyric acid (0.6 μM; 20 min) reflects the time-dependent increase in intracellular reactive oxygen species in CCRF-CEM cells following stimulation with exogenous H2O2 (980 μM)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa
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Concentration:50 μM
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Incubation Time:10 min
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Result:Enhanced the cell-killing effect of 5 μM R8-PAD, reducing cell viability to approximately 20%.
Chemical Information
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CAS No. 3443-45-6
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Appearance Solid
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Molecular Weight 288.35
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Formula C20H16O2
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Color Light yellow to light brown
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SMILES
C1=CC2=C3C(=C1)C=CC4=C(CCCC(=O)O)C=CC(=C43)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (346.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (286 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4680 mL | 17.3400 mL | 34.6801 mL | 86.7002 mL |
| 5 mM | 0.6936 mL | 3.4680 mL | 6.9360 mL | 17.3400 mL | |
| 10 mM | 0.3468 mL | 1.7340 mL | 3.4680 mL | 8.6700 mL | |
| 15 mM | 0.2312 mL | 1.1560 mL | 2.3120 mL | 5.7800 mL | |
| 20 mM | 0.1734 mL | 0.8670 mL | 1.7340 mL | 4.3350 mL | |
| 25 mM | 0.1387 mL | 0.6936 mL | 1.3872 mL | 3.4680 mL | |
| 30 mM | 0.1156 mL | 0.5780 mL | 1.1560 mL | 2.8900 mL | |
| 40 mM | 0.0867 mL | 0.4335 mL | 0.8670 mL | 2.1675 mL | |
| 50 mM | 0.0694 mL | 0.3468 mL | 0.6936 mL | 1.7340 mL | |
| 60 mM | 0.0578 mL | 0.2890 mL | 0.5780 mL | 1.4450 mL | |
| 80 mM | 0.0434 mL | 0.2168 mL | 0.4335 mL | 1.0838 mL | |
| 100 mM | 0.0347 mL | 0.1734 mL | 0.3468 mL | 0.8670 mL |
1-Pyrenebutyric acid Related Classifications
HY-W103047 Related Classifications
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