5-A-RU-PABC-Val-Cit-Fmoc

Name: 5-A-RU-PABC-Val-Cit-Fmoc
Brand: MedChemExpress
SKU: HY-131296
Rating: 4.5 (1 reviews)

Cat. No.: HY-131296 Purity: 98.85%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-A-RU-PABC-Val-Cit-Fmoc is the proagent of 5-A-RU. 5-A-RU, a precursor of bacterial Riboflavin, is a mucosal-associated invariant T (MAIT) cells activator. 5-A-RU forms potent MAIT-activating antigens via non-enzymatic reactions with small molecules, such as glyoxal and methylglyoxal, which are derived from other metabolic pathways.

For research use only. We do not sell to patients.

5-A-RU-PABC-Val-Cit-Fmoc Chemical Structure

CAS No. : 2677841-58-4

Size	Price	Stock	Quantity
5 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE 5-A-RU-PABC-Val-Cit-Fmoc

•Research Square Preprint. 2023 Aug 30.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5-A-RU-PABC-Val-Cit-Fmoc is the proagent of 5-A-RU^[1]. 5-A-RU, a precursor of bacterial Riboflavin, is a mucosal-associated invariant T (MAIT) cells activator. 5-A-RU forms potent MAIT-activating antigens via non-enzymatic reactions with small molecules, such as glyoxal and methylglyoxal, which are derived from other metabolic pathways^[2]^[3]^[4].

In Vitro

When added to MAIT cell cultures, 5-A-RU-PABC-Val-Cit-Fmoc (Compound 10) induces higher levels of mucosal-associated invariant T (MAIT) cell activation than 5-A-RU alone^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

903.93

Formula

C₄₃H₅₃N₉O₁₃

CAS No.

2677841-58-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(C=C1)COC(NC2=C(NC(NC2=O)=O)NC[C@@H]([C@@H]([C@@H](CO)O)O)O)=O)[C@@H](NC([C@@H](NC(OCC3C4=C(C5=C3C=CC=C5)C=CC=C4)=O)C(C)C)=O)CCCNC(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 150 mg/mL (165.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1063 mL	5.5314 mL	11.0628 mL
5 mM	0.2213 mL	1.1063 mL	2.2126 mL
10 mM	0.1106 mL	0.5531 mL	1.1063 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.75 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3.75 mg/mL (4.15 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.75 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.85%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (259 KB) HNMR (283 KB) LCMS (115 KB)

Handling Instructions (2659 KB)

References

[1]. Joshua Lange, et al. The Chemical Synthesis, Stability, and Activity of MAIT Cell Prodrug Agonists That Access MR1 in Recycling Endosomes. ACS Chem Biol. 2020 Feb 21;15(2):437-445. [Content Brief]

[2]. Corbett AJ, et al. T-cell activation by transitory neo-antigens derived from distinct microbial pathways. Nature. 2014 May 15;509(7500):361-5. [Content Brief]

[3]. Eckle SB, et al. Recognition of Vitamin B Precursors and Byproducts by Mucosal Associated Invariant T Cells. J Biol Chem. 2015 Dec 18;290(51):30204-11. [Content Brief]

[4]. Soudais C, et al. In Vitro and In Vivo Analysis of the Gram-Negative Bacteria-Derived Riboflavin PrecursorDerivatives Activating Mouse MAIT Cells. J Immunol. 2015 May 15;194(10):4641-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1063 mL	5.5314 mL	11.0628 mL	27.6570 mL
	5 mM	0.2213 mL	1.1063 mL	2.2126 mL	5.5314 mL
	10 mM	0.1106 mL	0.5531 mL	1.1063 mL	2.7657 mL
	15 mM	0.0738 mL	0.3688 mL	0.7375 mL	1.8438 mL
	20 mM	0.0553 mL	0.2766 mL	0.5531 mL	1.3829 mL
	25 mM	0.0443 mL	0.2213 mL	0.4425 mL	1.1063 mL
	30 mM	0.0369 mL	0.1844 mL	0.3688 mL	0.9219 mL
	40 mM	0.0277 mL	0.1383 mL	0.2766 mL	0.6914 mL
	50 mM	0.0221 mL	0.1106 mL	0.2213 mL	0.5531 mL
	60 mM	0.0184 mL	0.0922 mL	0.1844 mL	0.4610 mL
	80 mM	0.0138 mL	0.0691 mL	0.1383 mL	0.3457 mL
	100 mM	0.0111 mL	0.0553 mL	0.1106 mL	0.2766 mL