5-A-RU-PABC-Val-Cit-Fmoc
Based on 8 publication(s) in Google Scholar
5-A-RU-PABC-Val-Cit-Fmoc is the proagent of 5-A-RU. 5-A-RU, a precursor of bacterial Riboflavin, is a mucosal-associated invariant T (MAIT) cells activator. 5-A-RU forms potent MAIT-activating antigens via non-enzymatic reactions with small molecules, such as glyoxal and methylglyoxal, which are derived from other metabolic pathways.
For research use only. We do not sell to patients.
- Purity: 98.98%
- CAS No.: 2677841-58-4
- Formula: C43H53N9O13
- Molecular Weight:903.93
-
Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-A-RU-PABC-Val-Cit-Fmoc
More- Proc Natl Acad Sci U S A. 2026 Jun 16;123(24):e2601925123. [Abstract]
- PLoS Pathog. 2025 Aug 4;21(8):e1013342. [Abstract]
- Oral Dis. 2025 Jan;31(1):148-159. [Abstract]
- bioRxiv. 2025 Nov 29.
- bioRxiv. 2025 Oct 14.
- bioRxiv. 2025 Jun 26:2025.06.23.660389. [Abstract]
- bioRxiv. 2025 May 19:2025.05.14.654109. [Abstract]
- Res Sq. 2023 Aug 30:rs.3.rs-3154465. [Abstract]
Biological Activity
When added to MAIT cell cultures, 5-A-RU-PABC-Val-Cit-Fmoc (Compound 10) induces higher levels of mucosal-associated invariant T (MAIT) cell activation than 5-A-RU alone[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2677841-58-4
-
Appearance Solid
-
Molecular Weight 903.93
-
Formula C43H53N9O13
-
Color White to off-white
-
SMILES
O=C(NC1=CC=C(C=C1)COC(NC2=C(NC(NC2=O)=O)NC[C@@H]([C@@H]([C@@H](CO)O)O)O)=O)[C@@H](NC([C@@H](NC(OCC3C4=C(C5=C3C=CC=C5)C=CC=C4)=O)C(C)C)=O)CCCNC(N)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
Proc Natl Acad Sci U S A
Avoidance of MAIT cells is an essential determinant of Listeria monocytogenes pathogenesis. [Abstract]2026 Jun 16;123(24):e2601925123. PMID: 42258727 -
PLoS Pathog
2025 Aug 4;21(8):e1013342. PMID: 40758737 -
Oral Dis
Keratinocytes stimulate MAIT cells to produce granzyme B via MR1 and cytokines in oral lichen planus. [Abstract]2025 Jan;31(1):148-159. PMID: 38937944 -
-
-
bioRxiv
Synaptotagmin 1 and Synaptotagmin 7 promote MR1-mediated presentation of Mycobacterium tuberculosis antigens. [Abstract]2025 Jun 26:2025.06.23.660389. PMID: 40667066 -
bioRxiv
Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens. [Abstract]2025 May 19:2025.05.14.654109. PMID: 40475583 -
Res Sq
2023 Aug 30:rs.3.rs-3154465. PMID: 37693580
Solvent & Solubility
DMSO : 150 mg/mL (165.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.75 mg/mL (4.15 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3.75 mg/mL (4.15 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (281 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Joshua Lange, et al. The Chemical Synthesis, Stability, and Activity of MAIT Cell Prodrug Agonists That Access MR1 in Recycling Endosomes. ACS Chem Biol. 2020 Feb 21;15(2):437-445. [Content Brief]
[2]. Corbett AJ, et al. T-cell activation by transitory neo-antigens derived from distinct microbial pathways. Nature. 2014 May 15;509(7500):361-5. [Content Brief]
[3]. Eckle SB, et al. Recognition of Vitamin B Precursors and Byproducts by Mucosal Associated Invariant T Cells. J Biol Chem. 2015 Dec 18;290(51):30204-11. [Content Brief]
[4]. Soudais C, et al. In Vitro and In Vivo Analysis of the Gram-Negative Bacteria-Derived Riboflavin PrecursorDerivatives Activating Mouse MAIT Cells. J Immunol. 2015 May 15;194(10):4641-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1063 mL | 5.5314 mL | 11.0628 mL | 27.6570 mL |
| 5 mM | 0.2213 mL | 1.1063 mL | 2.2126 mL | 5.5314 mL | |
| 10 mM | 0.1106 mL | 0.5531 mL | 1.1063 mL | 2.7657 mL | |
| 15 mM | 0.0738 mL | 0.3688 mL | 0.7375 mL | 1.8438 mL | |
| 20 mM | 0.0553 mL | 0.2766 mL | 0.5531 mL | 1.3829 mL | |
| 25 mM | 0.0443 mL | 0.2213 mL | 0.4425 mL | 1.1063 mL | |
| 30 mM | 0.0369 mL | 0.1844 mL | 0.3688 mL | 0.9219 mL | |
| 40 mM | 0.0277 mL | 0.1383 mL | 0.2766 mL | 0.6914 mL | |
| 50 mM | 0.0221 mL | 0.1106 mL | 0.2213 mL | 0.5531 mL | |
| 60 mM | 0.0184 mL | 0.0922 mL | 0.1844 mL | 0.4610 mL | |
| 80 mM | 0.0138 mL | 0.0691 mL | 0.1383 mL | 0.3457 mL | |
| 100 mM | 0.0111 mL | 0.0553 mL | 0.1106 mL | 0.2766 mL |
5-A-RU-PABC-Val-Cit-Fmoc Related Classifications
HY-131296 Related Classifications
Powered by Bioz