1. Protein Tyrosine Kinase/RTK
  2. FLT3
  3. 5'-Fluoroindirubinoxime

5'-Fluoroindirubinoxime (Synonyms: 5'-FIO)

Cat. No.: HY-103464
Handling Instructions

5'-Fluoroindirubinoxime (5’-FIO, compound 13), an Indirubin (HY-N0117) derivative, is a potent FLT3 inhibitor, with an IC50 of 15 nM.

For research use only. We do not sell to patients.

5'-Fluoroindirubinoxime Chemical Structure

5'-Fluoroindirubinoxime Chemical Structure

CAS No. : 861214-33-7

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Description

5'-Fluoroindirubinoxime (5’-FIO, compound 13), an Indirubin (HY-N0117) derivative, is a potent FLT3 inhibitor, with an IC50 of 15 nM[1].

IC50 & Target

15 nM (FLT3)[1].

In Vitro

5'-Fluoroindirubinoxime (5’-FIO, compound 13) exhibits IC50 values of 1.53 μM and 1.27 μM for VEGFR2 and Aurora A, respectively[1].
5'-Fluoroindirubinoxime (5’-FIO) exhibits IC50 values of 12.2 μM, 2.1 μM, 3.4 μM and 5.1 μM in A549, SNU-638, HT-1080 and RK3E-ras cancer cells, respectively. 5'-Fluoroindirubinoxime (5’-FIO) induces the apoptosis in RK3E-ras cells[2].

In Vivo

5'-Fluoroindirubinoxime (5’-FIO, 10 μmol/L/100 μL (~2.95 mg/mL) every other day beginning on day 6, S.C..) exhibits significant anti-tumor activity in rats[2].

Animal Model: Rat tumor model based RK3E-ras cells[2].
Dosage: S.C..
Administration: 10 μmol/L/100 μL (~2.95 mg/mL) every other day beginning on day 6.
Result: Effectively inhibited tumor growth.
Molecular Weight

295.27

Formula

C₁₆H₁₀FN₃O₂

CAS No.

861214-33-7

SMILES

O=C1NC2=C(C=C(F)C=C2)/C1=C3NC4=C(C=CC=C4)C\3=N/O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (282.22 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3867 mL 16.9337 mL 33.8673 mL
5 mM 0.6773 mL 3.3867 mL 6.7735 mL
10 mM 0.3387 mL 1.6934 mL 3.3867 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (7.04 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

5'-Fluoroindirubinoxime5'-FIOFLT3Cluster of differentiation antigen 135CD135Fms like tyrosine kinase 3A549SNU-638HT-1080RK3E-rasInhibitorinhibitorinhibit

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5'-Fluoroindirubinoxime
Cat. No.:
HY-103464
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