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  2. Azide
  3. 7-Azido-4-methylcoumarin

7-Azido-4-methylcoumarin is a fluorescent hydrogen sulfide (H2S) probe. 7-Azido-4-methylcoumarin serves as a highly sensitive assay for cystathionine β-synthase activity.

For research use only. We do not sell to patients.

7-Azido-4-methylcoumarin Chemical Structure

7-Azido-4-methylcoumarin Chemical Structure

CAS No. : 95633-27-5

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5 mg USD 86 In-stock
10 mg USD 130 In-stock
25 mg USD 215 In-stock
50 mg USD 310 In-stock
100 mg USD 460 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

7-Azido-4-methylcoumarin is a fluorescent hydrogen sulfide (H2S) probe. 7-Azido-4-methylcoumarin serves as a highly sensitive assay for cystathionine β-synthase activity[1].

Molecular Weight

201.18

Formula

C10H7N3O2

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

[N-]=[N+]=NC1=CC(OC(C=C2C)=O)=C2C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (248.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9707 mL 24.8534 mL 49.7067 mL
5 mM 0.9941 mL 4.9707 mL 9.9413 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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Concentration (final)

C2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.9707 mL 24.8534 mL 49.7067 mL 124.2668 mL
5 mM 0.9941 mL 4.9707 mL 9.9413 mL 24.8534 mL
10 mM 0.4971 mL 2.4853 mL 4.9707 mL 12.4267 mL
15 mM 0.3314 mL 1.6569 mL 3.3138 mL 8.2845 mL
20 mM 0.2485 mL 1.2427 mL 2.4853 mL 6.2133 mL
25 mM 0.1988 mL 0.9941 mL 1.9883 mL 4.9707 mL
30 mM 0.1657 mL 0.8284 mL 1.6569 mL 4.1422 mL
40 mM 0.1243 mL 0.6213 mL 1.2427 mL 3.1067 mL
50 mM 0.0994 mL 0.4971 mL 0.9941 mL 2.4853 mL
60 mM 0.0828 mL 0.4142 mL 0.8284 mL 2.0711 mL
80 mM 0.0621 mL 0.3107 mL 0.6213 mL 1.5533 mL
100 mM 0.0497 mL 0.2485 mL 0.4971 mL 1.2427 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
7-Azido-4-methylcoumarin
Cat. No.:
HY-119323
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