8-Azido-ATP trisodium solution (100 mM)  (Synonyms: 8-Azidoadenosine 5'-triphosphate trisodium; 8-N3-ATP trisodium)

8-Azido-ATP (8-N3-ATP) trisodium, a photoreactable nucleotide analog, is useful for the identification of proteins, such as DNA-dependent RNA polymerase. 8-Azido-ATP trisodium is a click chemistry reagent that contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. 8-Azido-ATP trisodium can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups^[1][2].

614.14

C₁₀H₁₂N₈Na₃O₁₃P₃

Liquid

Colorless to light yellow

O[C@H]1[C@@H](O)[C@H](N2C(N=[N+]=[N-])=NC3=C(N=CN=C32)N)O[C@@H]1COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, 2 years

• [1]. S Valenzuela, et al. Photoaffinity labeling of rotavirus VP1 with 8-azido-ATP: identification of the viral RNA polymerase. J Virol. 1991 Jul;65(7):3964-7.  [Content Brief]

  [2]. K Tanabe, et al. Direct photoaffinity labeling of the Kir6.2 subunit of the ATP-sensitive K+ channel by 8-azido-ATP. J Biol Chem. 1999 Feb 12;274(7):3931-3.  [Content Brief]