8-Azido-ATP
8-Azido-ATP, a photoreactable nucleotide analog, is useful for the identification of proteins, such as DNA-dependent RNA polymerase. 8-Azido-ATP is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- CAS No.: 53696-59-6
- Formula: C10H15N8O13P3
- Molecular Weight:548.19
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
8-Azido-ATP can be used to identify the viral RNA polymerase on the basis of the ability of the analog to inhibit transcription activity associated with rotavirus particles on exposure to UV light[1].
8-Azido-ATP (azido-ATP) decreases viral transcription in a dose-dependent manner[1].
8-Azido-ATP blocks Kir6.2ΔC26 currents rather less potently than ATP, half-maximal inhibition (Ki) occurring at 2.8 ± 0.4 mM (n = 6) compared with 172 ± 7 mM for ATP (n = 6). The Hill coefficients were 0.9 ± 0.2 for 8-azido-ATP and 1.3 ± 0.1 for ATP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 53696-59-6
-
Molecular Weight 548.19
-
Formula C10H15N8O13P3
-
SMILES
O[C@H]1[C@@H](O)[C@H](N2C(N=[N+]=[N-])=NC3=C(N=CN=C32)N)O[C@@H]1COP(O)(OP(OP(O)(O)=O)(O)=O)=O
-
Synonyms
8-Azidoadenosine 5'-triphosphate; 8-N3-ATP
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
[1]. S Valenzuela, et al. Photoaffinity labeling of rotavirus VP1 with 8-azido-ATP: identification of the viral RNA polymerase. J Virol. 1991 Jul;65(7):3964-7. [Content Brief]
[2]. K Tanabe, et al. Direct photoaffinity labeling of the Kir6.2 subunit of the ATP-sensitive K+ channel by 8-azido-ATP. J Biol Chem. 1999 Feb 12;274(7):3931-3. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
8-Azido-ATP Related Classifications
HY-134320 Related Classifications
Powered by Bioz