1. Cell Cycle/DNA Damage
    Cytoskeleton
    Antibody-drug Conjugate/ADC Related
  2. Microtubule/Tubulin
    ADC Cytotoxin

Ansamitocin P-3 (Synonyms: Antibiotic C 15003P3; Maytansinol butyrate; C15003P3)

Cat. No.: HY-15739 Purity: >98.0%
Data Sheet SDS Handling Instructions

Ansamitocin P-3 is a microtubule inhibitor. Ansamitocin P-3 is a macrocyclic antitumor antibiotic.

For research use only. We do not sell to patients.
Ansamitocin P-3 Chemical Structure

Ansamitocin P-3 Chemical Structure

CAS No. : 66584-72-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO $182 In-stock
5 mg $130 In-stock
10 mg $190 In-stock
50 mg $450 In-stock
100 mg $600 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Other Forms of Ansamitocin P-3:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Ansamitocin P-3 is a microtubule inhibitor. Ansamitocin P-3 is a macrocyclic antitumor antibiotic.

IC50 & Target

Microtubule[1]

In Vitro

Ansamitocin P3 potently inhibits the proliferation of MCF-7, HeLa, EMT-6/AR1 and MDA-MB-231 cells in culture with a half-maximal inhibitory concentration of 20±3, 50±0.5, 140±17, and 150±1.1 pM, respectively. Further, Ansamitocin P3 is found to bind to purified tubulin in vitro with a dissociation constant (Kd) of 1.3±0.7 µM. The binding of Ansamitocin P3 induces conformational changes in tubulin. Ansamitocin P3 inhibits the proliferation of MCF-7, HeLa, EMT-6/AR1 and MDA-MB-231 cells in culture in a concentration dependent manner. Flow cytometric analysis of PI-stained cells suggests that Ansamitocin P3 inhibits the cell cycle progression of MCF-7 cells in G2/M phase. For example, 26, 50 and 70% of the cells are found to be in G2/M phase in the absence and presence of 50 and 100 pM Ansamitocin P3, respectively[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.5745 mL 7.8723 mL 15.7446 mL
5 mM 0.3149 mL 1.5745 mL 3.1489 mL
10 mM 0.1574 mL 0.7872 mL 1.5745 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

Ansamitocin P3 is dissolved in DMSO and stored, and then diluted with appropriate medium (DMSO 0.1%) before use[2].

MCF-7, EMT-6/AR1, HeLa and MDA-MB-231 cells are seeded in 96 well plates. Subsequently, cells are incubated with vehicle (0.1% DMSO) or different concentrations (1-1000 pM) of Ansamitocin P3 for 48 h in MCF-7 cells and 24 h for EMT-6/AR1, HeLa and MDA-MB-231 cells, respectively. The half maximal inhibitory concentration of cell proliferation (IC50) for Ansamitocin P3 is determined by sulforhodamine B assay. Four independent experiments are carried out in MCF-7 cells and three independent sets of experiments are performed in EMT-6/AR1, HeLa and MDA-MB-231 cells[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

635.14

Formula

C₃₂H₄₃ClN₂O₉

CAS No.

66584-72-3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
Ansamitocin P-3
Cat. No.:
HY-15739
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