BB-Cl-Amidine

Name: BB-Cl-Amidine
Brand: MedChemExpress
SKU: HY-111347
Rating: 5.0 (11 reviews)

Cat. No.: HY-111347 Pureza: 98.19% ee.: 100.00%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

BB-Cl-Amidine is a peptidylarginine deminase (PAD) inhibitor.

Para uso exclusivo en investigación. No vendemos a pacientes.

BB-Cl-Amidine Estructura química

No. CAS : 1802637-39-3

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 11 publication(s) in Google Scholar

Other Forms of BB-Cl-Amidine:

BB-Cl-Amidine hydrochloride In-stock
BB-Cl-Amidine TFA Obtener un presupuesto

11 Publications Citing Use of MCE BB-Cl-Amidine

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

BB-Cl-Amidine is a peptidylarginine deminase (PAD) inhibitor.

IC₅₀ & Target

PAD^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
U2OS	EC₅₀	8.8 μM Compound: 22; BB-Cl amidine	Cytotoxicity against human U2OS cells after 72 hrs by XTT assay Cytotoxicity against human U2OS cells after 72 hrs by XTT assay	[PMID: 28505447]
U2OS	IC₅₀	8.8 μM Compound: 15	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34520958]

In Vivo

Treatment with BB-Cl-amidine subtly reduces splenomegaly in MRL/lpr mice, while there is a trend towards increased circulating levels of anti-NET antibodies with PAD inhibitor treatment. However, neither PAD inhibitor affected body weight or total IgG levels. Indeed, treatment with both Cl-amidine and BB-Cl-amidine significantly improves endothelium-dependent vasorelaxation. The BB-Cl-amidine group also shows a strong trend towards downregulation of IRGs. Treatment with either Cl-amidine or BB-Cl-amidine significantly improves muzzle alopecia, in many cases preventing it entirely^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	MRL/lpr mice^[1].
Dosage:	1 mg/kg.
Administration:	Subcutaneous injection daily from 8 to 14 weeks of age.
Result:	Significantly improved endothelium-dependent vasorelaxation and showed a strong trend towards downregulation of IRGs.

Peso molecular

459.97

Fòrmula

C₂₆H₂₆ClN₅O

No. CAS

1802637-39-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(C2=CC=CC=C2)C=C1)N[C@H](C3=NC4=CC=CC=C4N3)CCCNC(CCl)=N

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (217.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1741 mL	10.8703 mL	21.7405 mL
5 mM	0.4348 mL	2.1741 mL	4.3481 mL
10 mM	0.2174 mL	1.0870 mL	2.1741 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 98.19% ee.: 100.00%

Select Batch:

Ficha de datos (273 KB) SDS (393 KB)

COA (248 KB) HNMR (299 KB) RP-HPLC (251 KB) NP-HPLC (132 KB) MS (69 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Knight JS, et al. Peptidylarginine deiminase inhibition disrupts NET formation and protects against kidney, skin and vascular disease in lupus-prone MRL/lpr mice. Ann Rheum Dis. 2015 Dec;74(12):2199-206. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1741 mL	10.8703 mL	21.7405 mL	54.3514 mL
	5 mM	0.4348 mL	2.1741 mL	4.3481 mL	10.8703 mL
	10 mM	0.2174 mL	1.0870 mL	2.1741 mL	5.4351 mL
	15 mM	0.1449 mL	0.7247 mL	1.4494 mL	3.6234 mL
	20 mM	0.1087 mL	0.5435 mL	1.0870 mL	2.7176 mL
	25 mM	0.0870 mL	0.4348 mL	0.8696 mL	2.1741 mL
	30 mM	0.0725 mL	0.3623 mL	0.7247 mL	1.8117 mL
	40 mM	0.0544 mL	0.2718 mL	0.5435 mL	1.3588 mL
	50 mM	0.0435 mL	0.2174 mL	0.4348 mL	1.0870 mL
	60 mM	0.0362 mL	0.1812 mL	0.3623 mL	0.9059 mL
	80 mM	0.0272 mL	0.1359 mL	0.2718 mL	0.6794 mL
	100 mM	0.0217 mL	0.1087 mL	0.2174 mL	0.5435 mL

BB-Cl-Amidine Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BB-Cl-Amidine1802637-39-3Protein Arginine DeiminasePeptidylarginine DeiminaseInhibitorinhibitorinhibit

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BB-Cl-Amidine

Revisión del cliente

Other Forms of BB-Cl-Amidine:

BB-Cl-Amidine Related Antibodies

11 Publications Citing Use of MCE BB-Cl-Amidine

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

BB-Cl-Amidine Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

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SAFETY DATA SHEET (SDS)

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