BB-Cl-Amidine hydrochloride
Based on 11 publication(s) in Google Scholar
BB-Cl-Amidine hydrochloride is a peptidylarginine deminase (PAD) inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.47%
- CAS No.: 2436747-41-8
- Formula: C26H27Cl2N5O
- Molecular Weight:496.43
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BB-Cl-Amidine hydrochloride
More- Nat Cell Biol. 2021 Oct;23(10):1060-1072. [Abstract]
- Nat Commun. 2024 Sep 3;15(1):7654. [Abstract]
- Adv Sci (Weinh). 2025 Aug 20:e11873. [Abstract]
- Mol Med. 2025 Feb 5;31(1):46. [Abstract]
- Acta Neuropathol Commun. 2025 Feb 18;13(1):33. [Abstract]
- Int Immunopharmacol. 2024 Nov 5;143(Pt 3):113534. [Abstract]
- Oncol Rep. 2023 Jul;50(1):146. [Abstract]
- J Antibiot (Tokyo). 2025 Oct 9. [Abstract]
- Cell Physiol Biochem. 2024 Feb 19;58(1):63-82. [Abstract]
- bioRxiv. 2026 Jan 21:2026.01.20.700682. [Abstract]
- Res Sq. 2024 Jun 04.
Biological Activity
PAD[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MRL/lpr mice[1].
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Dosage:1 mg/kg.
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Administration:Subcutaneous injection daily from 8 to 14 weeks of age.
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Result:Significantly improved endothelium-dependent vasorelaxation and showed a strong trend towards downregulation of IRGs.
Chemical Information
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CAS No. 2436747-41-8
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Appearance Solid
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Molecular Weight 496.43
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Formula C26H27Cl2N5O
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Color White to off-white
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SMILES
O=C(C1=CC=C(C2=CC=CC=C2)C=C1)N[C@H](C3=NC4=CC=CC=C4N3)CCCNC(CCl)=N.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (11)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
2021 Oct;23(10):1060-1072. PMID: 34616019 -
Nat Commun
2024 Sep 3;15(1):7654. PMID: 39227578 -
Adv Sci (Weinh)
Succinate Dehydrogenase Subunit A (SDHA) Mediated Microglia Extracellular Traps Formation Participating in Cerebral Ischemic Reperfusion Injury. [Abstract]2025 Aug 20:e11873. PMID: 40832885 -
Mol Med
Platelet-derived HMGB1 induces NETosis, exacerbating brain damage in the photothrombotic stroke model. [Abstract]2025 Feb 5;31(1):46. PMID: 39910417 -
Acta Neuropathol Commun
Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms. [Abstract]2025 Feb 18;13(1):33. PMID: 39966968 -
Int Immunopharmacol
HSYA ameliorates venous thromboembolism by depleting the formation of TLR4/NF-κB pathway-dependent neutrophil extracellular traps. [Abstract]2024 Nov 5;143(Pt 3):113534. PMID: 39504860 -
Oncol Rep
Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice. [Abstract]2023 Jul;50(1):146. PMID: 37326108 -
J Antibiot (Tokyo)
Antibacterial effects of BB-Cl-Amidine against multidrug-resistant Gram-positive pathogens via membrane disruption. [Abstract]2025 Oct 9. PMID: 41068498 -
Cell Physiol Biochem
Peptidylarginine Deiminases Inhibitors Decrease Endothelial Cells Angiogenic Potential by Affecting Akt Signaling and the Expression and Secretion of Angiogenic Factors. [Abstract]2024 Feb 19;58(1):63-82. PMID: 38374715 -
bioRxiv
Reciprocal regulation between the protein arginine deiminases and mSWI/SNF chromatin remodelers controls skeletal muscle differentiation and regeneration. [Abstract]2026 Jan 21:2026.01.20.700682. PMID: 41659480 -
Solvent & Solubility
DMSO : 50 mg/mL (100.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (10.07 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.0144 mL | 10.0719 mL | 20.1438 mL | 50.3596 mL |
| 5 mM | 0.4029 mL | 2.0144 mL | 4.0288 mL | 10.0719 mL | |
| 10 mM | 0.2014 mL | 1.0072 mL | 2.0144 mL | 5.0360 mL | |
| DMSO | 15 mM | 0.1343 mL | 0.6715 mL | 1.3429 mL | 3.3573 mL |
| 20 mM | 0.1007 mL | 0.5036 mL | 1.0072 mL | 2.5180 mL | |
| 25 mM | 0.0806 mL | 0.4029 mL | 0.8058 mL | 2.0144 mL | |
| 30 mM | 0.0671 mL | 0.3357 mL | 0.6715 mL | 1.6787 mL | |
| 40 mM | 0.0504 mL | 0.2518 mL | 0.5036 mL | 1.2590 mL | |
| 50 mM | 0.0403 mL | 0.2014 mL | 0.4029 mL | 1.0072 mL | |
| 60 mM | 0.0336 mL | 0.1679 mL | 0.3357 mL | 0.8393 mL | |
| 80 mM | 0.0252 mL | 0.1259 mL | 0.2518 mL | 0.6295 mL | |
| 100 mM | 0.0201 mL | 0.1007 mL | 0.2014 mL | 0.5036 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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