1. Epigenetics
  2. Protein Arginine Deiminase

BB-Cl-Amidine 

Cat. No.: HY-111347 Purity: 99.98%
Handling Instructions

BB-Cl-Amidine is a peptidylarginine deminase (PAD) inhibitor.

For research use only. We do not sell to patients.

BB-Cl-Amidine Chemical Structure

BB-Cl-Amidine Chemical Structure

CAS No. : 1802637-39-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 96 In-stock
Estimated Time of Arrival: December 31
1 mg USD 50 In-stock
Estimated Time of Arrival: December 31
5 mg USD 95 In-stock
Estimated Time of Arrival: December 31
10 mg USD 170 In-stock
Estimated Time of Arrival: December 31
25 mg USD 360 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

BB-Cl-Amidine is a peptidylarginine deminase (PAD) inhibitor.

IC50 & Target

PAD[1].

In Vitro

Cl-amidine and BB-Cl-amidine show similar in vitro potencies and selectivities; however, the cellular potency of BB-Cl-amidine is increased by more than 20-fold, evidenced by decreased EC50 values obtained from viability studies with U2OS osteosarcoma cells (8.8±0.6 μM vs >200 μM for Cl-amidine). Cl-amidine and BB-Cl-amidine significantly inhibit NET formation by MRL/lpr neutrophils[1].

In Vivo

Treatment with BB-Cl-amidine subtly reduces splenomegaly in MRL/lpr mice, while there is a trend towards increased circulating levels of anti-NET antibodies with PAD inhibitor treatment. However, neither PAD inhibitor affected body weight or total IgG levels. Indeed, treatment with both Cl-amidine and BB-Cl-amidine significantly improves endothelium-dependent vasorelaxation. The BB-Cl-amidine group also shows a strong trend towards downregulation of IRGs. Treatment with either Cl-amidine or BB-Cl-amidine significantly improves muzzle alopecia, in many cases preventing it entirely[1].

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (271.76 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1741 mL 10.8703 mL 21.7405 mL
5 mM 0.4348 mL 2.1741 mL 4.3481 mL
10 mM 0.2174 mL 1.0870 mL 2.1741 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

References
Animal Administration
[1]

Mice[1]
MRL/lpr mice are treated with either Cl-amidine (Cl-am; 10 mg/kg/day) or BB-Cl-amidine (BB-Cl-am; 1 mg/kg/day) by daily subcutaneous injection from 8 to 14 weeks of age[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

459.97

Formula

C₂₆H₂₆ClN₅O

CAS No.

1802637-39-3

SMILES

O=C(C1=CC=C(C2=CC=CC=C2)C=C1)N[[email protected]](C3=NC4=CC=CC=C4N3)CCCNC(CCl)=N

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

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Product Name:
BB-Cl-Amidine
Cat. No.:
HY-111347
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BB-Cl-Amidine

Cat. No.: HY-111347