N-(2-Chloro-6-methylphenyl)-N'-4-pyridinylurea
Based on 1 Customer Validation
N-(2-Chloro-6-methylphenyl)-N'-4-pyridinylurea is an anticonvulsant agent with potential for the treatment of generalized tonic-clonic and partial seizures.
For research use only. We do not sell to patients.
- Purity : 99.73%
- CAS No.: 97627-24-2
- Formula: C13H12ClN3O
- Molecular Weight:261.71
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 97627-24-2
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Appearance Solid
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Molecular Weight 261.71
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Formula C13H12ClN3O
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Color White to off-white
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SMILES
O=C(NC1=CC=NC=C1)NC2=C(C)C=CC=C2Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (477.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Animal Administration[1]
Mice: Five mice are tested at each of three doses (30,100, and 300 mg/kg) and three times (0.5, 2, and 4 h). The mice are subjected to electrical current delivered through ear clips for 0.2 s (90 mA, 1-ms monophasic pulses at 100 Hz). This current strength is approximately 4 times that required to produce seizures in 99% of mice and reliably produce seizures in 100% of control mice. Prevention of tonic hind limb extension is taken as an anticonvulsant effect. Behavioral side effects are measured in mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.8210 mL | 19.1051 mL | 38.2102 mL | 95.5256 mL |
| 5 mM | 0.7642 mL | 3.8210 mL | 7.6420 mL | 19.1051 mL | |
| 10 mM | 0.3821 mL | 1.9105 mL | 3.8210 mL | 9.5526 mL | |
| 15 mM | 0.2547 mL | 1.2737 mL | 2.5473 mL | 6.3684 mL | |
| 20 mM | 0.1911 mL | 0.9553 mL | 1.9105 mL | 4.7763 mL | |
| 25 mM | 0.1528 mL | 0.7642 mL | 1.5284 mL | 3.8210 mL | |
| 30 mM | 0.1274 mL | 0.6368 mL | 1.2737 mL | 3.1842 mL | |
| 40 mM | 0.0955 mL | 0.4776 mL | 0.9553 mL | 2.3881 mL | |
| 50 mM | 0.0764 mL | 0.3821 mL | 0.7642 mL | 1.9105 mL | |
| 60 mM | 0.0637 mL | 0.3184 mL | 0.6368 mL | 1.5921 mL | |
| 80 mM | 0.0478 mL | 0.2388 mL | 0.4776 mL | 1.1941 mL | |
| 100 mM | 0.0382 mL | 0.1911 mL | 0.3821 mL | 0.9553 mL |
N-(2-Chloro-6-methylphenyl)-N'-4-pyridinylurea Related Classifications
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