1. NF-κB
    Immunology/Inflammation
    Apoptosis
  2. NF-κB
    Interleukin Related
    TNF Receptor
    COX
  3. Neochlorogenic acid

Neochlorogenic acid (Synonyms: trans-5-O-Caffeoylquinic acid)

Cat. No.: HY-N0722 Purity: 99.46%
Handling Instructions

Neochlorogenic acid is a natural polyphenolic compound found in dried fruits and other plants. Neochlorogenic acid inhibits the production of TNF-α and IL-1β. Neochlorogenic acid suppresses iNOS and COX-2 protein expression. Neochlorogenic acid also inhibits phosphorylated NF-κB p65 and p38 MAPK activation.

For research use only. We do not sell to patients.

Neochlorogenic acid Chemical Structure

Neochlorogenic acid Chemical Structure

CAS No. : 906-33-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 165 In-stock
Estimated Time of Arrival: December 31
5 mg USD 150 In-stock
Estimated Time of Arrival: December 31
10 mg USD 240 In-stock
Estimated Time of Arrival: December 31
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Other Forms of Neochlorogenic acid:

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Description

Neochlorogenic acid is a natural polyphenolic compound found in dried fruits and other plants. Neochlorogenic acid inhibits the production of TNF-α and IL-1β. Neochlorogenic acid suppresses iNOS and COX-2 protein expression. Neochlorogenic acid also inhibits phosphorylated NF-κB p65 and p38 MAPK activation.

IC50 & Target[1]

p65

 

IL-1β

 

TNF-α

 

COX-2

 

In Vitro

Neochlorogenic acid (NCA) shows a reduction of lipopolysaccharide (LPS)-induced NO production by suppressing iNOS and COX-2 protein expression and production of pro-inflammatory cytokines, such as TNF-α and IL-1β, in BV2 microglia cells. In addition, phosphorylated p38 MAPK and NF-κB p65 are also inhibited by Neochlorogenic acid in activated microglia. iNOS and COX-2 levels are increased in LPS-induced BV2 cells, but this increase is significantly inhibited after treatment with 50 and 100 μM Neochlorogenic acid[1].

Molecular Weight

354.31

Formula

C₁₆H₁₈O₉

CAS No.

906-33-2

SMILES

O=C([[email protected]]1(O)C[[email protected]@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[[email protected]@H](O)[[email protected]](O)C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 11 mg/mL (31.05 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8224 mL 14.1119 mL 28.2239 mL
5 mM 0.5645 mL 2.8224 mL 5.6448 mL
10 mM 0.2822 mL 1.4112 mL 2.8224 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Mouse BV2 microglial cells are maintained in DMEM, supplemented with 5 % FBS and 1 % antibiotic–antimycotic in a humidified incubator with 5 % CO2 at 37°C. Neochlorogenic acid and Dexamethasone as positive control are dissolved in DMSO to a final concentration of 10 mM for the stock solution. Treatments with LPS and/or Neochlorogenic acid are carried out under serum-free conditions. Effects of Neochlorogenic acid are measured on cell viability in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. The cells are treated with or without LPS (4 μg/ml) and Neochlorogenic acid (10, 50, and 100 μM) for 24 h. Dexamethasone (10 μM) is used for positive control. Cell viability is confirmed by the MTT assay. The medium was removed from the wells, MTT was added, and the samples were then incubated for 3 h at 37°C. The formazan crystals were dissolved by adding DMSO, and the absorbance values were measured at 540 nm using a microplate reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

Neochlorogenic acidtrans-5-O-Caffeoylquinic acidNF-κBInterleukin RelatedTNF ReceptorCOXNuclear factor-κBNuclear factor-kappaBILTumor Necrosis Factor ReceptorTNFRCyclooxygenaseInhibitorinhibitorinhibit

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Neochlorogenic acid
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HY-N0722
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