1. Metabolic Enzyme/Protease
  2. MMP

Prinomastat (Synonyms: AG3340; KB-R9896)

Cat. No.: HY-12170 Purity: 95.03%
Handling Instructions

Prinomastat is a broad spectrum MMP inhibitor with IC50s of 79, 6.3 and 5.0 nM for MMP-1, MMP-3 and MMP-9, respectively.

For research use only. We do not sell to patients.

Prinomastat Chemical Structure

Prinomastat Chemical Structure

CAS No. : 192329-42-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 251 In-stock
Estimated Time of Arrival: December 31
5 mg USD 228 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

View All MMP Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Prinomastat is a broad spectrum MMP inhibitor with IC50s of 79, 6.3 and 5.0 nM for MMP-1, MMP-3 and MMP-9, respectively.

IC50 & Target

IC50: 79 nM (MMP-1), 6.3 nM (MMP-3), 5.0 nM (MMP-9)[1]

In Vitro

Co-culture of L/Wnt3a cells and CT7 cells increases the Topflash activity in CT7 cells and this effect is suppressed in the presence of Prinomastat (AG3340). The effect of Wnt1 on the cellular distribution of vimentin is reversed in the presence of Prinomastat in C57MG/Wnt1 cells. Casein gel zymography and western blot analysis of the supernatant of C57MG/Wnt1 cells demonstrate a significant decrease in the presence of MMP-3 in the supernatant of cells treated with Prinomastat or rTIMP-2 that is consistent with these inhibitors inhibiting Wnt1-induced MMP-3 production[3].

In Vivo

Treatment of HaCaT-ras A-5RT3 tumor xenografts with Prinomastat (Ag3340) for 6 days strongly reduces tumor invasion and vascularization. The mean vessel diameter, analyzed by vessel size imaging with MRI, remains constant in untreated animals (day 0: 53±10 μm, day 6: 57±20 μm), but significantly increases in response to Prinomastat treatment (prior to therapy, day 0: 40±10 μm, after therapy, day 6: 70±10 μm; p<0.05). Prinomastat treatment markedly reduces the expression of murine VEGF-A in the stroma of the HaCaT-ras A-5RT3 tumors[2].

Clinical Trial
References
Cell Assay
[3]

Cells are seeded into 100-mm plates and cultured to confluence in the presence or absence of 10 µg/mL Prinomastat (AG3340). Cells are washed in PBS, resuspended in 70% ethanol and stored overnight at 4°C. Cells are incubated with 20 µg/mL RNase A for 30 min at 37°C, centrifuged, and resuspended in PBS containing 40 µg/mL propidium iodide. The analysis is performed on an cell sorter software[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

A total of 14 nude mice are examined using ultrasound (US), starting on day 21 after subcutaneous (s.c.) injection of tumor cells. After the US examination, animals receive either 110 μL MMP inhibitor Prinomastat (Ag3340, 150 mg/kg twice a day, i.p. for 6 days; n=7) or 110 μL NaCl solution (twice a day, i.p. for 6 days; control group, n=7). After 6 days, US examination is repeated. Magnetic resonance imaging (MRI) of treated and untreated animals is only performed at the second time point[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

423.51

Formula

C₁₈H₂₁N₃O₅S₂

CAS No.

192329-42-3

SMILES

O=C([[email protected]@H]1N(S(=O)(C2=CC=C(OC3=CC=NC=C3)C=C2)=O)CCSC1(C)C)NO

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: 95.03%

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Prinomastat
Cat. No.:
HY-12170
Quantity:

Prinomastat

Cat. No.: HY-12170