Ac-Lys(Ac)-D-Ala-D-Ala-OH 

Ac-Lys (Ac)-D-Ala-D-Ala-OH is a synthetic dipeptide analog that mimics the terminal structure of bacterial peptidoglycan chains. Ac-Lys (Ac)-D-Ala-D-Ala-OH serves as a molecular decoy to investigate the mechanism of action of Vancomycin-class antibiotics, and also acts as an affinity chromatography ligand for purifying proteins that bind to Vancomycin. Ac-Lys (Ac)-D-Ala-D-Ala-OH reduces the activity of Vancomycin against susceptible enterococci. Ac-Lys (Ac)-D-Ala-D-Ala-OH is applicable to the research of Vancomycin-resistant enterococcal infections^[1].

Ac-Lys (Ac)-D-Ala-D-Ala-OH (Fig3, A) (20:1, 5:1 peptide:Vancomycin molar ratio; pre-incubation for 1 h, bacterial incubation for 24 h) completely antagonizes the activity of 8 μg/mL and 32 μg/mL Vancomycin (HY-B0671) against Enterococcus faecalis TX2016 at a peptide-to-Vancomycin molar ratio of 20:1, but shows no antagonistic activity at a molar ratio of 5:1^[1].
Ac-Lys (Ac)-D-Ala-D-Ala-OH (8 μg/mL vancomycin; 1 hour pre-incubation, 24 hour bacterial incubation) antagonizes the activity of 8 μg/mL vancomycin against Enterococcus faecium TX2016, with an IC₅₀ of 137 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

372.42

C₁₆H₂₈N₄O₆

24570-39-6

Ac-{Lys(Ac)}-{d-Ala}-{d-Ala}

Ac-{Lys(Ac)}-{d-Ala}-{d-Ala}

[C@@H](C(N[C@@H](C(N[C@@H](C(O)=O)C)=O)C)=O)(CCCCNC(C)=O)NC(C)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mull RW, et al. Attenuating the Selection of Vancomycin Resistance Among Enterococci through the Development of Peptide-Based Vancomycin Antagonists. ACS Infect Dis. 2020;6(11):2913-2925.