2. Immunology/Inflammation
  3. Interleukin Related
  4. AMY109

AMY109 is an anti-human interleukin-8 (IL-8) monoclonal antibody, with IC50 values of 0.14 μg/mL and 0.37 μg/mL against human IL-8, a Ka of 36.8 pM for human IL-8, and a Ka of 380 pM for cynomolgus monkey IL-8.\nAMY109 binds to human and cynomolgus monkey IL-8 in a pH-dependent manner, inhibits IL-8-mediated activation of CXCR1 and CXCR2, and blocks the downstream biological activities of IL-8. AMY109 inhibits neutrophil recruitment to endometriotic lesions and suppresses monocyte chemoattractant protein-1 production by neutrophils. AMY109 is applicable to research related to endometriosis.

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AMY109

AMY109 Chemical Structure

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AMY109 Related Antibodies

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Description

AMY109 is an anti-human interleukin-8 (IL-8) monoclonal antibody, with IC50 values of 0.14 μg/mL and 0.37 μg/mL against human IL-8, a Ka of 36.8 pM for human IL-8, and a Ka of 380 pM for cynomolgus monkey IL-8.\nAMY109 binds to human and cynomolgus monkey IL-8 in a pH-dependent manner, inhibits IL-8-mediated activation of CXCR1 and CXCR2, and blocks the downstream biological activities of IL-8. AMY109 inhibits neutrophil recruitment to endometriotic lesions and suppresses monocyte chemoattractant protein-1 production by neutrophils. AMY109 is applicable to research related to endometriosis[1].

Species Reactivity

Human
In Vitro

AMY109 (pH 7.4; pH 5.8) exhibits high binding affinity to human IL-8 and weaker but measurable binding affinity to cynomolgus monkey IL-8 under neutral conditions, with pH-dependent dissociation from human IL-8 at endosomal pH[1].
AMY109 potently neutralizes IL-8-dependent activation of both CXCR1 and CXCR2 in cell-based assays[1].
AMY109 (600 ng/mL IL-8) effectively inhibits IL-8-induced chemotaxis of both human and cynomolgus monkey neutrophils[1].
AMY109 inhibits both IL-8-induced human neutrophil migration and IL-8-stimulated MCP-1 production by human neutrophils[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AMY109 Related Antibodies
In Vivo

AMY109 (2-10 mg/kg; subcutaneous injection; every 4 weeks; 6 months) stabilizes disease progression, reduces nodular lesion volume, induces atrophic changes in endometriotic lesions, decreases fibrosis, and reduces postsurgical adhesions in cynomolgus monkeys with surgically induced endometriosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Macaca fascicularis (female, reproductive age 8 to 14 years old, surgically induced endometriosis)[1]
Dosage: 2 mg/kg (maintenance dose; preceded by a 3 mg/kg loading dose); 10 mg/kg
Administration: subcutaneous injection; every 4 weeks; 6 months
Result: Maintained modified r-ASRM score stable, while it significantly increased in vehicle-treated monkeys.
Reduced relative volume of nodular lesions.
Induced atrophy of endometrial epithelium in 55% of evaluable sites (2 mg/kg) and 54% of evaluable sites (10 mg/kg).
Induced atrophy/decreased stromal cells in 33% of evaluable sites (2 mg/kg) and 60% of evaluable sites (10 mg/kg).
Decreased fibrotic interstitium in 34% of evaluable sites (2 mg/kg) and 61% of evaluable sites (10 mg/kg).
Reduced hemosiderin deposition compared to vehicle.
Produced marked reduction in postsurgical incision adhesions in 6 of 7 monkeys treated with 10 mg/kg.
Achieved greater lesion volume reduction in monkeys without antidrug antibodies (ADAs) than those with ADAs.
Gene ID

3576  [NCBI]

Accession
Target

CXCL8/IL-8
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

[AMY109]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AMY109 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMY109AMY 109AMY-109Interleukin RelatedILCXCR1endometriosisendometriotic lesionscynomolgus monkeyinterleukin-8neutrophilsIL-8monocyte chemoattractant protein-1neutrophil recruitmentCXCR2Inhibitorinhibitorinhibit

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