Tin(II) chloride, 98%
Based on 1 Customer Validation
Tin(II) chloride, 98% (Stannous chloride) is a biochemical reagent with genotoxicity, mutagenicity, mitotic recombinogenicity, and reactive oxygen species-inducing activity.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 7772-99-8
- Formula: Cl2Sn
- Molecular Weight:189.62
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Tin(II) chloride, 98% (1.00-5.00 mM; 20 min) induces mutagenic activity in Salmonella typhimurium TA102, with a mutagenic index of 2.0 at 5.00 mM and significant dose-dependent increases in revertant colony counts at concentrations ≥1.25 mM[1].
Tin(II) chloride, 98% (0.125-1.25 mM; 20 min) induces mutagenic activity in oxidant-sensitive Escherichia coli IC203, with a mutagenic index of 2.0 at 1.25 mM and significant dose-dependent increases in revertant colony counts at concentrations ≥1.00 mM[1].
Tin(II) chloride, 98% (25 mM; 90 min) reduces survival of Saccharomyces cerevisiae stationary-phase cells, with recombinogenic repair-deficient rad52Δ cells showing the highest sensitivity among isogenic repair mutants, indicating recombinogenic repair mechanisms are most critical for repairing Sn2+-induced DNA damage[1].
Tin(II) chloride, 98% (25-75 mM; 20-60 min) acts as a mutagen in Saccharomyces cerevisiae, inducing dose-dependent reversion of histidine, lysine, and putative frameshift mutant alleles in haploid and diploid stationary-phase cells, with higher activity for the his1-798 allele[1].
Tin(II) chloride, 98% (25-75 mM; 1-60 min) induces significant intra-genic mitotic recombination (gene conversion) in diploid Saccharomyces cerevisiae XS2316 cells at 50 mM, with minimal to no induction of inter-genic reciprocal recombination at tested concentrations (25, 50, 75 mM)[1].
Tin(II) chloride, 98% (25 mM; up to 60 min) induces toxicity in stationary-phase Saccharomyces cerevisiae that is mitigated by superoxide dismutases (especially cytosolic Sod1p), but not by catalase[1].
Tin(II) chloride, 98% reduces survival of both stationary-phase and exponentially growing Saccharomyces cerevisiae XV185-14c cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7772-99-8
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Appearance Solid
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Molecular Weight 189.62
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Formula Cl2Sn
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Color White to off-white
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SMILES
Cl[Sn]Cl
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Synonyms
Stannous chloride, 98%; Tin(II) chloride, anhydrous, 98%; Anhydrous stannous chloride, 98%
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (527.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (26.37 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (26.37 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (782 KB)
- English - EN (782 KB)
- Français - FR (782 KB)
- Deutsch - DE (782 KB)
- Norwegian - NO (782 KB)
- Español - ES (782 KB)
- Swedish - SV (782 KB)
- Italian - IT (782 KB)
- Korean - KR (782 KB)
- Portuguese - PT (782 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2737 mL | 26.3685 mL | 52.7371 mL | 131.8426 mL |
| 5 mM | 1.0547 mL | 5.2737 mL | 10.5474 mL | 26.3685 mL | |
| 10 mM | 0.5274 mL | 2.6369 mL | 5.2737 mL | 13.1843 mL | |
| 15 mM | 0.3516 mL | 1.7579 mL | 3.5158 mL | 8.7895 mL | |
| 20 mM | 0.2637 mL | 1.3184 mL | 2.6369 mL | 6.5921 mL | |
| 25 mM | 0.2109 mL | 1.0547 mL | 2.1095 mL | 5.2737 mL | |
| 30 mM | 0.1758 mL | 0.8790 mL | 1.7579 mL | 4.3948 mL | |
| 40 mM | 0.1318 mL | 0.6592 mL | 1.3184 mL | 3.2961 mL | |
| 50 mM | 0.1055 mL | 0.5274 mL | 1.0547 mL | 2.6369 mL | |
| 60 mM | 0.0879 mL | 0.4395 mL | 0.8790 mL | 2.1974 mL | |
| 80 mM | 0.0659 mL | 0.3296 mL | 0.6592 mL | 1.6480 mL | |
| 100 mM | 0.0527 mL | 0.2637 mL | 0.5274 mL | 1.3184 mL |
Tin(II) chloride, 98% Related Classifications
HY-Y0481 Related Classifications
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- Tin(II) chloride, 98%
- 7772-99-8
- Stannous chloride, 98%
- Tin(II) chloride, anhydrous, 98%
- Anhydrous stannous chloride, 98%
- Biochemical Assay Reagents
- Reactive Oxygen Species (ROS)
- Saccharomyces cerevisiae
- mutagenicity
- superoxide dismutases
- Salmonella typhimurium
- reactive oxygen species
- genotoxicity
- cytotoxicity
- Escherichia coli
- DNA damage
- mitotic recombinogenicity
- Inhibitor
- inhibitor
- inhibit