Cat. No.: HY-107859

Tris(2-chloroethyl) phosphate

(Synonyms: TCEP)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Tris(2-chloroethyl) phosphate (TCEP) is a widely used organic phosphorus flame retardant, mainly used as a plasticizer. Tris(2-chloroethyl) phosphate has orally active hepatotoxicity, inducing an increase in reactive oxygen species (ROS) and calcium ions (Ca²⁺) influx, a decrease in mitochondrial membrane potential (△Ψm), and causing DNA damage and cell apoptosis. Tris(2-chloroethyl) phosphate directly binds to FXR, inducing obesity and the formation of fatty liver in mice. Chloroethyl) phosphate activates the TLR4/NF-κB pathway, triggering liver inflammation.

For research use only. We do not sell to patients.

Purity: 97.07%
CAS No.: 115-96-8
Formula: C₆H₁₂Cl₃O₄P
Molecular Weight:285.49

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Tris(2-chloroethyl) phosphate Chemical Structure

CAS No.: 115-96-8

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Biological Activity
- Description
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All TNF Receptor Isoforms :

All Calcium Channel Isoforms :

All NF-κB Isoforms :

All Interleukin Related Isoforms :

All Toll-like Receptor (TLR) Isoforms :

All DNA/RNA Synthesis Isoforms :

In Vitro

Tris(2-chloroethyl) phosphate (100-400 μM, 3 days) induces the death of HepG2 cells by disrupting mitochondrial function and the integrity of lysosomal membranes^[3].
Tris(2-chloroethyl) phosphate (100-400 μM, 3 days) causes dose-dependent DNA strand breaks, induces ROS/NO bursts, Ca²⁺ influx and △Ψm collapse in HepG2 cells, and triggers apoptosis^[3].
Tris(2-chloroethyl) phosphate (100 μM, 3 days) upregulates oncogenes such as IGFBP5 and STMN1, and downregulates tumor suppressor genes such as SNAI1 in HepG2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[3]

Cell Line:

HepG2 cells
Concentration:

100, 200 and 400 μM
Incubation Time:

3 d
Result:

Significantly induced apoptosis, the proportion of the SubG1 peak reached 65.96% in the 400 μM group.

In Vivo

Tris(2-chloroethyl) phosphate (0.5-5 μg/L, for 28 days) exerts hepatotoxic impacts on zebrafish by disrupting hypothalamic-pituitary-thyroid and gut−liver axes^[1].
Tris(2-chloroethyl) phosphate (20-60 mg/kg, i.g., once daily for 9 weeks) induces obesity and hepatic steatosis in mice through the FXR signaling pathway^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Toxicity test models model established in male CD-1 mice (4-6 weeks old, 20-25 g)^[2]
Dosage:

20 and 60 mg/kg
Administration:

Oral gavage (i.g.), once daily for 9 weeks
Result:

Promoted weight gain, expansion of adipose tissue and elevation of serum lipids.
Directly combined and inhibited FXR, thereby down-regulating SHP, activating the SREBP1c/PPARγ lipogenesis pathway, and inhibiting fatty acid oxidation mediated by PPARα.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 115-96-8
Appearance Liquid (Density: 1.425 g/cm³)
Molecular Weight 285.49
Formula C₆H₁₂Cl₃O₄P
Color Colorless to light yellow
SMILES

O=P(OCCCl)(OCCCl)OCCCl
Synonyms

TCEP
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

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Journal Impact Factor

Chem Eng J

2025 Oct 15;524:169625.
Adv Healthc Mater

Oral Administration of Trifluoroacetyl Chitosan-Encapsulated Redshifted Immunofluorophore for NIR-II Bioimaging of Colorectal Metastases. [Abstract]

2025 Oct 15:e01877. PMID: 41097850
Int J Biol Macromol

Bacterial cellulose membrane integrating phase-transited lysozyme nanofilm loaded with biguanides for dressing treatment of atopic dermatitis. [Abstract]

2025 Aug 21;323(Pt 1):147047. PMID: 40848785
Neural Regen Res

Disulfidptosis contributes to rotenone-induced dopaminergic neuron damage. [Abstract]

2025 Oct 30. PMID: 41169225
Commun Chem

Stepped collisional energy improves protein acylation identification by eliminating positional bias of cyclic immonium ions. [Abstract]

2025 Dec 9. PMID: 41366033
Res Sq

2025 May 06.

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (700.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5027 mL

17.5138 mL

35.0275 mL

5 mM

0.7005 mL

3.5027 mL

7.0055 mL

10 mM

0.3503 mL

1.7514 mL

3.5027 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tian D, et al. Tris(2-chloroethyl) Phosphate Exerts Hepatotoxic Impacts on Zebrafish by Disrupting Hypothalamic-Pituitary-Thyroid and Gut-Liver Axes. Environ Sci Technol. 2023 Jun 20;57(24):9043-9054. [Content Brief]

[2]. Yang D, et al. Tris (2-chloroethyl) phosphate (TCEP) induces obesity and hepatic steatosis via FXR-mediated lipid accumulation in mice: Long-term exposure as a potential risk for metabolic diseases. Chem Biol Interact. 2022 Aug 25;363:110027. [Content Brief]

[3]. M Al-Salem A, et al. Tris(2-chloroethyl) Phosphate (TCEP) Elicits Hepatotoxicity by Activating Human Cancer Pathway Genes in HepG2 Cells. Toxics. 2020 Nov 20;8(4):109.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5027 mL	17.5137 mL	35.0275 mL	87.5687 mL
	5 mM	0.7005 mL	3.5027 mL	7.0055 mL	17.5137 mL
	10 mM	0.3503 mL	1.7514 mL	3.5027 mL	8.7569 mL
	15 mM	0.2335 mL	1.1676 mL	2.3352 mL	5.8379 mL
	20 mM	0.1751 mL	0.8757 mL	1.7514 mL	4.3784 mL
	25 mM	0.1401 mL	0.7005 mL	1.4011 mL	3.5027 mL
	30 mM	0.1168 mL	0.5838 mL	1.1676 mL	2.9190 mL
	40 mM	0.0876 mL	0.4378 mL	0.8757 mL	2.1892 mL
	50 mM	0.0701 mL	0.3503 mL	0.7005 mL	1.7514 mL
	60 mM	0.0584 mL	0.2919 mL	0.5838 mL	1.4595 mL
	80 mM	0.0438 mL	0.2189 mL	0.4378 mL	1.0946 mL
	100 mM	0.0350 mL	0.1751 mL	0.3503 mL	0.8757 mL

Keywords

Tris(2-chloroethyl) phosphate

6 Cited Publications

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Tris(2-chloroethyl) phosphate

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Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

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Publications Citing Use of MedChemExpress (MCE) Tris(2-chloroethyl) phosphate

All TNF Receptor Isoforms

All Calcium Channel Isoforms

All NF-κB Isoforms

All Interleukin Related Isoforms

All Toll-like Receptor (TLR) Isoforms

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tris(2-chloroethyl) phosphate Related Classifications

HY-107859 Related Classifications