2. Others Membrane Transporter/Ion Channel NF-κB Metabolic Enzyme/Protease Immunology/Inflammation Apoptosis Neuronal Signaling Cell Cycle/DNA Damage
  3. SOD TNF Receptor Interleukin Related NF-κB Calcium Channel DNA/RNA Synthesis Reactive Oxygen Species (ROS) Environmental Pollutants FXR Toll-like Receptor (TLR) Apoptosis
  4. Tris(2-chloroethyl) phosphate

Tris(2-chloroethyl) phosphate  (Synonyms: TCEP)

Cat. No.: HY-107859 Purity: 97.07%
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Tris(2-chloroethyl) phosphate (TCEP) is a widely used organic phosphorus flame retardant, mainly used as a plasticizer. Tris(2-chloroethyl) phosphate has orally active hepatotoxicity, inducing an increase in reactive oxygen species (ROS) and calcium ions (Ca²⁺) influx, a decrease in mitochondrial membrane potential (△Ψm), and causing DNA damage and cell apoptosis. Tris(2-chloroethyl) phosphate directly binds to FXR, inducing obesity and the formation of fatty liver in mice. Chloroethyl) phosphate activates the TLR4/NF-κB pathway, triggering liver inflammation.

For research use only. We do not sell to patients.

Tris(2-chloroethyl) phosphate

Tris(2-chloroethyl) phosphate Chemical Structure

CAS No. : 115-96-8

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10 mM * 1 mL in DMSO
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Based on 6 publication(s) in Google Scholar

Other Forms of Tris(2-chloroethyl) phosphate:

Tris(2-chloroethyl) phosphate Related Antibodies

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Description

Tris(2-chloroethyl) phosphate (TCEP) is a widely used organic phosphorus flame retardant, mainly used as a plasticizer. Tris(2-chloroethyl) phosphate has orally active hepatotoxicity, inducing an increase in reactive oxygen species (ROS) and calcium ions (Ca²⁺) influx, a decrease in mitochondrial membrane potential (△Ψm), and causing DNA damage and cell apoptosis. Tris(2-chloroethyl) phosphate directly binds to FXR, inducing obesity and the formation of fatty liver in mice. Chloroethyl) phosphate activates the TLR4/NF-κB pathway, triggering liver inflammation[1][2][3].

In Vitro

Tris(2-chloroethyl) phosphate (100-400 μM, 3 days) induces the death of HepG2 cells by disrupting mitochondrial function and the integrity of lysosomal membranes[3].
Tris(2-chloroethyl) phosphate (100-400 μM, 3 days) causes dose-dependent DNA strand breaks, induces ROS/NO bursts, Ca²⁺ influx and △Ψm collapse in HepG2 cells, and triggers apoptosis[3].
Tris(2-chloroethyl) phosphate (100 μM, 3 days) upregulates oncogenes such as IGFBP5 and STMN1, and downregulates tumor suppressor genes such as SNAI1 in HepG2 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tris(2-chloroethyl) phosphate Related Antibodies

Apoptosis Analysis[3]

Cell Line: HepG2 cells
Concentration: 100, 200 and 400 μM
Incubation Time: 3 d
Result: Significantly induced apoptosis, the proportion of the SubG1 peak reached 65.96% in the 400 μM group.
In Vivo

Tris(2-chloroethyl) phosphate (0.5-5 μg/L, for 28 days) exerts hepatotoxic impacts on zebrafish by disrupting hypothalamic-pituitary-thyroid and gut−liver axes[1].
Tris(2-chloroethyl) phosphate (20-60 mg/kg, i.g., once daily for 9 weeks) induces obesity and hepatic steatosis in mice through the FXR signaling pathway[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Toxicity test models model established in male CD-1 mice (4-6 weeks old, 20-25 g)[2]
Dosage: 20 and 60 mg/kg
Administration: Oral gavage (i.g.), once daily for 9 weeks
Result: Promoted weight gain, expansion of adipose tissue and elevation of serum lipids.
Directly combined and inhibited FXR, thereby down-regulating SHP, activating the SREBP1c/PPARγ lipogenesis pathway, and inhibiting fatty acid oxidation mediated by PPARα.
Clinical Trial
Molecular Weight

285.49

Formula

C6H12Cl3O4P
CAS No.
Appearance

Liquid (Density: 1.425 g/cm3)

Color

Colorless to light yellow

SMILES

O=P(OCCCl)(OCCCl)OCCCl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (700.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5027 mL 17.5138 mL 35.0275 mL
5 mM 0.7005 mL 3.5027 mL 7.0055 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5027 mL 17.5137 mL 35.0275 mL 87.5687 mL
5 mM 0.7005 mL 3.5027 mL 7.0055 mL 17.5137 mL
10 mM 0.3503 mL 1.7514 mL 3.5027 mL 8.7569 mL
15 mM 0.2335 mL 1.1676 mL 2.3352 mL 5.8379 mL
20 mM 0.1751 mL 0.8757 mL 1.7514 mL 4.3784 mL
25 mM 0.1401 mL 0.7005 mL 1.4011 mL 3.5027 mL
30 mM 0.1168 mL 0.5838 mL 1.1676 mL 2.9190 mL
40 mM 0.0876 mL 0.4378 mL 0.8757 mL 2.1892 mL
50 mM 0.0701 mL 0.3503 mL 0.7005 mL 1.7514 mL
60 mM 0.0584 mL 0.2919 mL 0.5838 mL 1.4595 mL
80 mM 0.0438 mL 0.2189 mL 0.4378 mL 1.0946 mL
100 mM 0.0350 mL 0.1751 mL 0.3503 mL 0.8757 mL
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Tris(2-chloroethyl) phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tris(2-chloroethyl)115-96-8TCEPSODTNF ReceptorInterleukin RelatedNF-κBCalcium ChannelDNA/RNA SynthesisReactive Oxygen Species (ROS)Environmental PollutantsFXRToll-like Receptor (TLR)ApoptosisSuperoxide DismutaseTumor Necrosis Factor ReceptorTNFRILNuclear factor-κBNuclear factor-kappaBCa2+ channelsCa channelsEnvironmental ContaminantsNR1H4Inhibitorinhibitorinhibit

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