Oral Administration of Trifluoroacetyl Chitosan-Encapsulated Redshifted Immunofluorophore for NIR-II Bioimaging of Colorectal Metastases

  • Adv Healthc Mater. 2025 Oct 15:e01877. doi: 10.1002/adhm.202501877.
Yibing Shi  1 ,  Xiaoyi Zhu  1 ,  Qingyuan Xu  1 ,  Yanling Wu  1 ,  Yishuo Sun  2 ,  Xin Zhao  3 ,  Yulong Liu  1 ,  Wei Feng  2 ,  Youhua Xie  1  4 ,  Tianlei Ying  1 ,  Zhenlin Yang  1  5
Affiliations
  • 1. Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Key Laboratory of Lung Inflammation and Injury, Department of Pulmonary Medicine, Zhongshan Hospital, Shanghai Engineering Research Center for Synthetic Immunology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
  • 2. Department of Chemistry, Fudan University, Shanghai, 200433, China.
  • 3. Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, Shanghai, 200032, China.
  • 4. Children's Hospital, Fudan University, Shanghai, 201102, China.
  • 5. Greater Bay Area Institute of Precision Medicine (Guangzhou), Fudan University, Shanghai, 200032, China.
PMID: 41097850 DOI: 10.1002/adhm.202501877
Abstract

The oral administration of immunofluorophores has been limited by gastrointestinal degradation. To address this, a novel immunofluorophore (ICGM-B9-FCS), based on a single-domain antibody-dye targeting the CEACAM5 antigen, is coated with trifluoromethyl chitosan (5 mg kg-1) for NIR-II bioimaging of colorectal Cancer and its distant metastases. ICGM-B9-FCS demonstrates unique advantages over existing intraoperative tumor-guidance bioagents, including resistance to liver metabolism, well-retained bioaffinity (EC50 = 58.09 nmol L-1), enhanced relative quantum yield (up to 12 equivalents), significant redshifts in absorption (from 783 to 957 nm), effective oral delivery to colonic metastases, and in situ exploration of the capillary-lymphatic network transportation. These findings establish a regulatory pathway for modifying immunofluorophores as orally administered bioagents, offering innovative methods for preoperative diagnosis and intraoperative guidance in colorectal Cancer.

Keywords
NIR‐II bioimaging; fully human single domain antibody; large redshift; oral administration; trifluoroacetyl chitosan.
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