Anti-Ganglioside GD2 Antibody (14G2a)
Based on 1 Customer Validation
Anti-Ganglioside GD2 Antibody (14G2a) is a kind of mouse IgG2a κ chimeric antibody inhibitor, targeting to human Ganglioside GD2. Anti-Ganglioside GD2 Antibody (14G2a) can induce neuroblastoma cells autophagy and lysis. Anti-Ganglioside GD2 Antibody (14G2a) can be used for the research of cancer, such as neuroblastoma.
For research use only. We do not sell to patients.
- Purity : ≥95.0%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
Ganglioside GD2
In Vitro
Anti-Ganglioside GD2 Antibody (14G2a) (40 μg/mL, 48 h) induces autophagy in IMR 32 and CHP 134 neuroblastoma cells[1].
Anti-Ganglioside GD2 Antibody (14G2a) (1 μg/well) induces NMB-7 neuroblastoma target cells lysis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:IMR-32 neuroblastoma cells
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Concentration:40 μg/mL
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Incubation Time:48 h
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Result:Increased LC3A/B, ATG, caspase 3/7 PHLDA1 expression.
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Cell Line:IMR-32 and CHP 134 neuroblastoma cells
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Concentration:40 μg/mL
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Incubation Time:48 h
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Result:Increased autophagic flux producyion.
Increased number of autophagosomes.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NMB-7 neuroblastoma mice (BALB/c athymic (nu/nu) mice)[2]
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Dosage:200 μg
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Administration:Intravenously injection, at days 2, 5, 8, 11, 14, 17, 23, 26, 29, 32, 35, 38, 41, and 44
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Result:Had no detectable tumor 83 days after the initial tumor cell inoculation.
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vitro induction of apoptosis in GD2+ cells; in vivo inhibition of GD2+ tumor cell growth
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Ganglioside GD2 Antibody (14G2a)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Durbas M, et al. GD2 ganglioside-binding antibody 14G2a and specific aurora A kinase inhibitor MK-5108 induce autophagy in IMR-32 neuroblastoma cells. Apoptosis. 2018 Oct;23(9-10):492-511. [Content Brief]
[2]. Mujoo K, et al. Functional properties and effect on growth suppression of human neuroblastoma tumors by isotype switch variants of monoclonal antiganglioside GD2 antibody 14.18. Cancer Res. 1989 Jun 1;49(11):2857-61. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Ganglioside GD2 Antibody (14G2a) Related Classifications
HY-P990845 Related Classifications
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