Anti-Mouse CD28 Antibody (D665)
Anti-Mouse CD28 Antibody (D665) is a mouse-derived agonistic IgG1 κ type antibody, targeting to mouse CD28. Anti-Mouse CD28 Antibody (D665) induces the activation of T cells. Anti-Mouse CD28 Antibody (D665) can be used for the researches of neurological disease, inflammation, immunology and cancer, such as Parkinson disease (PD), arthritis and graft-versus-host disease (GVHD).
For research use only. We do not sell to patients.
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Mouse IgG1 kappa
Mouse
CD28
Anti-Mouse CD28 Antibody (D665) (100 μg, i.p., at days 7) reduces arthritis severity in glucose-6-phosphate isomerase (G6PI)-induced arthritis mice[2].
Anti-Mouse CD28 Antibody (D665) (250 μg, i.p., 3 days before isolation of splenic CD4+ cells) prevents graft-versus-host disease in CD4+ cells from C57BL/6 mice transferred BALB/c mice[3].
Anti-Mouse CD28 Antibody (D665) (250 μg, i.p., 3 days before isolation of the T cells from total splenocytes) prolongs survival in T cells transferred BCL lymphoma mice[3].
Anti-Mouse CD28 Antibody (D665) (50 μg/mouse, i.p., 3 days before tMCAO) increases regulatory T cells (Treg) and increases thrombo-inflammation and ischemic neurodegeneration in transient middle cerebral artery occlusion (tMCAO) mice models[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HαSyn-injected PD mice[1]
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Dosage:200 μg
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Administration:Intraperitoneally injection, at days 7
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Result:Increased Treg in cervical lymph nodes and spleen.
Showed a significant elevation of CD4+CD25+FoxP3+ Treg.
Revealed an expansion of Treg in brain.
Increased IL-10 levels in cervical lymph nodes and spleen.
Prolonged latency to fall from the rotarod.
Prevented the loss of dopaminergic and total Nissl+ neurons in the SN.
Reduced numbers of CD4+, CD8+ T cells and CD11b+ myeloid cells.
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Animal Model:Glucose-6-phosphate isomerase (G6PI)-induced arthritis mice[2]
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Dosage:100 μg
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Administration:Intraperitoneally injection, at days 7
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Result:Reduced arthritis severity scores.
Showed no infiltration of neutrophils, macrophages or lymphocytes into the joints and cartilage destruction.
Increased CD4+CD25+Foxp3+ Treg cells.
Reduced the percentage of Th cells producing TNF-α, IL-17 or RANKL.
Increased Treg cells in both spleens and lymph nodes and levels of CTLA4 expression and proliferation.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
in vivo T cell stimulation/activation; in vitro T cell stimulation/activation
Chemical Information
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Molecular Weight 150 kDa
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SMILES
[Anti-Mouse CD28 Antibody (D665)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Badr M, et al. Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice. J Neuroinflammation. 2022 Dec 31;19(1):319. [Content Brief]
[2]. Win SJ, et al. In vivo activation of Treg cells with a CD28 superagonist prevents and ameliorates chronic destructive arthritis in mice. Eur J Immunol. 2016 May;46(5):1193-202. [Content Brief]
[3]. Beyersdorf N, et al. Superagonistic CD28 stimulation of allogeneic T cells protects from acute graft-versus-host disease. Blood. 2009 Nov 12;114(20):4575-82. [Content Brief]
[4]. Schuhmann MK, et al. CD28 superagonist-mediated boost of regulatory T cells increases thrombo-inflammation and ischemic neurodegeneration during the acute phase of experimental stroke. J Cereb Blood Flow Metab. 2015 Jan;35(1):6-10. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD28 Antibody (D665) Related Classifications
HY-P990793 Related Classifications
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