2. Immunology/Inflammation
  3. Transmembrane Glycoprotein CD44
  4. Anti-Mouse/Human CD44 Antibody (IM7)

Anti-Mouse/Human CD44 Antibody (IM7) 

Cat. No.: HY-P99126 Purity: 99.07%
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Anti-Mouse/Human CD44 Antibody (IM7) is an anti-huamn and mouse CD44 IgG2b monoclonal antibody that recognizes a conserved epitope outside the HA-binding domain of the CD44 molecule. Anti-Mouse/Human CD44 Antibody (IM7) exerts a potent anti-inflammatory effect by inducing the shedding of cell surface CD44, significantly improving symptoms in mice with rheumatoid arthritis without affecting relevant immune responses. Anti-Mouse/Human CD44 Antibody (IM7) can be used for researches on inflammation conditions and cancer such as arthritis and osteosarcoma.

For research use only. We do not sell to patients.

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Customer Review

Based on 2 publication(s) in Google Scholar

Anti-Mouse/Human CD44 Antibody (IM7) Featured Recommendations:

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Top Publications Citing Use of Products

2 Publications Citing Use of MCE Anti-Mouse/Human CD44 Antibody (IM7)

In Vivo Efficacy Study
Histological Imaging/Staining
WB
RT-PCR
Flow Cytometry

    Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315.  [Abstract]

    Anti-Mouse/Human CD44 Antibody (IM7) (100 μL; i.p.; 7 d) significantly attenuated body weight loss, reduced disease activity index (DAI) scores, and partially restored colon length in UC (ulcerative colitis) mice.

    Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315.  [Abstract]

    Anti-Mouse/Human CD44 Antibody (IM7) (100 μL; i.p.; 7 d) repaired mucosal epithelial barriers, diminished inflammatory infiltration, and improved histopathology scores in colon tissues of UC (ulcerative colitis) mice.

    Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315.  [Abstract]

    Anti-Mouse/Human CD44 Antibody (IM7) (10 μg/mL) reduced CD44 protein levels and elevated ATP7A expression in BMDM-derived Ly6Chi macrophages.

    Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315.  [Abstract]

    Anti-Mouse/Human CD44 Antibody (IM7) (10 μg/mL) reduced mRNA levels of pro-inflammatory cytokines IL-6 and TNF-α in BMDM-derived Ly6Chi macrophages.

    Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315.  [Abstract]

    Anti-Mouse/Human CD44 Antibody (IM7) (10 μg/mL) diminished the ROS proportion in BMDM-derived Ly6Chi macrophages.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Anti-Mouse/Human CD44 Antibody (IM7) is an anti-huamn and mouse CD44 IgG2b monoclonal antibody that recognizes a conserved epitope outside the HA-binding domain of the CD44 molecule. Anti-Mouse/Human CD44 Antibody (IM7) exerts a potent anti-inflammatory effect by inducing the shedding of cell surface CD44, significantly improving symptoms in mice with rheumatoid arthritis without affecting relevant immune responses. Anti-Mouse/Human CD44 Antibody (IM7) can be used for researches on inflammation conditions and cancer such as arthritis and osteosarcoma[1][2][3].

    Isotype

    Rat IgG2b kappa
    Recommend Isotype Controls
    Species Reactivity

    Human,Mouse
    IC50 & Target

    CD44
    In Vitro

    Anti-Mouse/Human CD44 Antibody (IM7) (0.05-50 μg/mL, 24-72 h) significantly reduces cell activity of SW1353 cells in a time-dependent manner[1].
    Anti-Mouse/Human CD44 Antibody (IM7) (50 μg/mL, 72 h) significantly induces cell apoptosis of SW1353 cells[1].
    Anti-Mouse/Human CD44 Antibody (IM7) (12-20 h) inhibits the migration and adhesion of inflammatory cells by inducing CD44 shedding and blocking hyaluronic acid binding in white blood cells and synovial cells from arthritis mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Anti-Mouse/Human CD44 Antibody (IM7) Related Antibodies

    Cell Viability Assay[1]

    Cell Line: SW1353 cells
    Concentration: 0.05, 5 and 50 μg/mL
    Incubation Time: 24, 48 and 72 h
    Result: Significantly reduced the activity of SW1353 cells after 72 hours.
    In Vivo

    Anti-Mouse/Human CD44 Antibody (IM7) (50 and 100 μg, i.p., once daily, for 10 days) significantly alleviates symptoms of arthritis without significant side effects in BALB/c mice and DBA/1 mice with arthritis[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c mice with proteoglycan-arthritis and DBA/1 mice with collagen-arthritis[2]
    Dosage: Initial dose of 100 µg, maintenance dose of 50 µg
    Administration: Intraperitoneal injection (i.p.), once daily, for 10 days
    Result: Significantly reduced joint swelling within 24-36 hours.
    Restored normal joint function in mice after 8 days of treatment.
    Significantly reduced leukocyte infiltration in synovium.
    Prevented white blood cells from migrating to synovial tissue, but did not affect their homing to lymph nodes.
    Gene ID

    12505  [NCBI]

    Accession

    NP_001034240.1
    Application

    in vivo CD44 neutralization; in vitro CD44 neutralization
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    Molecular Weight

    150 kDa

    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Anti-Mouse/Human CD44 Antibody (IM7)]
    Shipping

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Biological Activity
    • Immobilized CD44 Protein, Mouse (HEK293, His, HY-P74291) can bind Anti-Mouse CD44 Antibody (IM7). The EC50 for this effect is 0.165 μg/mL.
    Purity & Documentation

    Purity: 99.07%

    SDS (251 KB)

    COA (232 KB) SDS-PAGE (67 KB) SEC-HPLC (133 KB)

    Inhibitory Antibodies User Guide (603 KB)
    References
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    • If you have published this work, please enter the PubMed ID.
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    Anti-Mouse/Human CD44 Antibody (IM7) Related Classifications

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    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Anti-Mouse/Human CD44 Antibody (IM7)Transmembrane GlycoproteinCD44In (Lu)-related protein p80Pgp-1/Ly-24ECMRIIIHUTCH-1inflammationcancerarthritisosteosarcomaSW1353Inhibitorinhibitorinhibit

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