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Osteopontin/OPN Protein, Mouse (HEK293, His)

5 Cited Publications
Customer Review

Based on 5 publication(s) in Google Scholar

Osteopontin/OPN Protein, a crucial non-collagenous bone protein, binds strongly to hydroxyapatite, integral to the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Moreover, as a cytokine, OPN efficiently induces interferon-gamma and interleukin-12 production while inhibiting interleukin-10, pivotal in promoting type I immunity.Osteopontin/OPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Osteopontin/OPN protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Osteopontin/OPN Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Osteopontin/OPN Protein, a crucial non-collagenous bone protein, binds strongly to hydroxyapatite, integral to the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Moreover, as a cytokine, OPN efficiently induces interferon-gamma and interleukin-12 production while inhibiting interleukin-10, pivotal in promoting type I immunity.Osteopontin/OPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Osteopontin/OPN protein, expressed by HEK293 , with C-His labeled tag.

Background

Osteopontin (OPN) protein, a vital non-collagenous bone protein, demonstrates a strong affinity for hydroxyapatite and serves as an integral component of the mineralized matrix. It likely plays a crucial role in facilitating interactions between cells and the extracellular matrix. Additionally, OPN acts as a cytokine, effectively stimulating the production of interferon-gamma and interleukin-12, while simultaneously suppressing the production of interleukin-10. These functions are essential in the pathway that promotes type I immunity.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 105 cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is <0.3 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1425 μg/mL, corresponding to a specific activity is 7017.5439 units/mg

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P10923 (L17-N294)

  • Gene ID
    20750  [NCBI]
  • Molecular Construction
    • N-term
    • OPN (L17-N294)
      Accession # P10923
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SPP1; Uropontin; Prev. BNSP; Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion Protein; Prev. OPN; CDNA FLJ54682, Highly Similar To Osteopontin; Osteopontin; Secreted Phosphoprotein 1 Variant 6; Lnc-PKD2-2-3; Bone Sialoprotein I; ETA-1

  • AA Sequence

    LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVN

  • Predicted Molecular Mass

    31.8 kDa

  • Molecular Weight

    Approximately 50-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1425 μg/mL, corresponding to a specific activity is 7017.5439 units/mg

  • 1/16

    Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]

    Customer Validation
     rmOPN (200 ng/mL, 30 min) was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. ELISAs were used to detected the concentration of IL-6 and TNF-α in cell supernatant of MH-s cells.

  • 1/16

    Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]

    Customer Validation
     rmOPN (200 ng/mL, 30 min) was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells.

  • 1/16

    Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]

    Customer Validation
     rmOPN (200 ng/mL, 30 min) was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells.

  • 1/16

    Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 May;36(19):e2311964. [Abstract]

    Customer Validation
     Heatmap and volcano plots of DEGs between control and SPP1 group in vitro.

  • Osteopontin/OPN Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Osteopontin/OPN Protein, Mouse (HEK293, His)
  • Osteopontin/OPN Protein, Mouse (HEK293, His) Product Image

  • Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]

    rmOPN (200 ng/mL, 30 min) was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. ELISAs were used to detected the concentration of IL-6 and TNF-α in cell supernatant of MH-s cells.

  • Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]

    rmOPN (200 ng/mL, 30 min) was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells.

  • Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]

    rmOPN (200 ng/mL, 30 min) was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells.

  • Osteopontin/OPN Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 May;36(19):e2311964. [Abstract]

    Heatmap and volcano plots of DEGs between control and SPP1 group in vitro.