Anti-Mouse CD8α Antibody (YTS169.4)

Cat. No.: HY-P991112 Purity: 98.99%: Data Sheet
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Anti-Mouse CD8α Antibody (YTS169.4) is an IgG2 monoclonal antibody targeting mouse CD8α. Anti-Mouse CD8α Antibody (YTS169.4) rapidly depletes or downregulates CD8⁺ T cells in the spleens of Biozzi AB/H mice. Anti-Mouse CD8α Antibody (YTS169.4) is applicable to research related to hepatitis B virus infection. Recommended isotype control: Rat IgG2b kappa, Isotype Control (HY-P990682).

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사이즈	재고	수량
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

Anti-Mouse CD8α Antibody (YTS169.4) is an IgG2 monoclonal antibody targeting mouse CD8α. Anti-Mouse CD8α Antibody (YTS169.4) rapidly depletes or downregulates CD8⁺ T cells in the spleens of Biozzi AB/H mice. Anti-Mouse CD8α Antibody (YTS169.4) is applicable to research related to hepatitis B virus infection^[1]^[2]. Recommended isotype control: Rat IgG2b kappa, Isotype Control (HY-P990682).

Isotype

Rat IgG2b kappa

Recommend Isotype Controls

Rat IgG2b kappa, Isotype Control

Species Reactivity

Mouse

In Vivo

Anti-Mouse CD8α Antibody (YTS169.4) (75-250 μg; i.p., i.c.; single injection) rapidly depletes peripheral CD8⁺ splenocytes but fails to inhibit the development, severity, or relapse of chronic relapsing experimental allergic encephalomyelitis in Biozzi AB/H mice, regardless of administration timing or route^[1].
Anti-Mouse CD8α Antibody (YTS169.4) (2 mg; i.v.; twice (3 days and 1 day prior to α-GalCer injection)), when co-administered alongside rat anti-mouse CD4 (YTS191.1) antibody, reduces intrahepatic CD8⁺ T cell populations to <1.5% of preinjection levels without affecting NKT or NK cell counts^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Biozzi AB/H (H-2^dq1)^[1]
Dosage:	250 μg (i.p. administration; splenocyte depletion; CREAE pre-acute, acute active, post-acute remission treatment); 75 μg (i.c. administration; CNS CREAE testing)
Administration:	i.p.; single injection (day 9 p.i., day 12 p.i., acute disease onset, day 27 p.i.); i.c.; single injection (day 12 p.i.)
Result:	Rapidly depleted CD8+ splenocytes to 0.1% within 24 hours post 250 μg i.p. injection, remained at 0.1% at 7 days, and recovered to 1.2% at 27 days. Failed to inhibit CREAE development, with 7/8 mice developing clinical CREAE (mean score 2.6, onset day 17.9) after day 9 p.i. pre-acute treatment, 13/14 mice developing clinical CREAE (mean score 3.0) after day 12 p.i. pre-acute treatment, and 6/6 mice developing clinical CREAE (mean score 2.8, onset day 16.5 ) after day 12 p.i. i.c. treatment. Failed to prevent CREAE relapse, with 6/8 mice relapsing (onset day 40.5) after day 12 p.i. pre-acute treatment, 8/9 mice relapsing (mean score 2.9, onset day 37.8) after day 27 p.i. post-acute remission treatment.

Animal Model:	HBV transgenic lineage 1.3.32, C57BL/6 × BALB/c F1 hybrid (male, 8-10 weeks old, matched for serum HBeAg levels)^[2]
Dosage:	2 mg
Administration:	i.v.; twice (3 days and 1 day prior to α-GalCer injection)
Result:	Reduced intrahepatic CD8⁺ T cell populations to <1.5% of preinjection levels. Left NKT cell and NK cell numbers unaffected.

Gene ID

12525 [NCBI]

Accession

P01731

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

분자량

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CD8a Antibody (YTS169.4)]

선적

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Biological Activity

Loaded Anti-Mouse CD8α Antibody (YTS169.4) on AHC2 biosensor, can bind Mouse CD8α with an affinity constant of 1.08E-09 M as determined in BLI assay.

순도&문서

Purity: 98.99%

Select Batch:

Data Sheet (262 KB) SDS (252 KB)

COA (233 KB) SDS-PAGE (78 KB) SEC-HPLC (86 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. O'Neill JK, et al. Control of immune-mediated disease of the central nervous system with monoclonal (CD4-specific) antibodies. J Neuroimmunol. 1993;45(1-2):1-14. [Content Brief]

[2]. Kakimi K, et al. Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J Exp Med. 2000;192(7):921-930. [Content Brief]