Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) 

Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) is a recombinant chimeric version of the original 10F.9G2 antibody (HY-P99145). The variable domain sequences are identical to the original 10F.9G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG1. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) blocks PD-1 signaling. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) can be used for the research of cancer.

Mouse IgG1 (D265A) kappa

Mouse IgG1 (D265A) kappa, Isotype Control

Mouse

PD-L1/B7-H1

60533  [NCBI]

Q9EP73

in vivo PD-L1 blockade; Immunofluorescence; Immunohistochemistry (frozen)*Flow cytometry*Western b

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

143.882 kDa

Liquid

Colorless to light yellow

[Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2)]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

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  Immobilized Mouse PDL1, His Tag can bind Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2). The EC₅₀ for this effect is 91.87 ng/mL.
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  Flow cytometric analysis of 1X10⁶ MC38 cells with Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) (HY-P990172, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 (D265A) kappa (HY-P99983, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).