2. Immunology/Inflammation
  3. PD-1/PD-L1
  4. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2)

Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) 

Cat. No.: HY-P990172 Pureté: 99.32%
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Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) is a recombinant chimeric version of the original 10F.9G2 antibody (HY-P99145). The variable domain sequences are identical to the original 10F.9G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG1. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) blocks PD-1 signaling. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) can be used for the research of cancer.

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Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) Related Antibodies

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Description

Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) is a recombinant chimeric version of the original 10F.9G2 antibody (HY-P99145). The variable domain sequences are identical to the original 10F.9G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG1. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) blocks PD-1 signaling. Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) can be used for the research of cancer.

Isotype

Mouse IgG1 (D265A) kappa
Recommend Isotype Controls
Species Reactivity

Mouse
IC50 & Target

PD-L1/B7-H1
Gene ID

60533  [NCBI]

Accession
Application

in vivo PD-L1 blockade; Immunofluorescence; Immunohistochemistry (frozen)*Flow cytometry*Western b
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Masse moléculaire

143.882 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2)]
Livraison

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized Mouse PDL1, His Tag can bind Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2). The EC50 for this effect is 91.87 ng/mL.
  • Flow cytometric analysis of 1X106 MC38 cells with Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) (HY-P990172, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 (D265A) kappa (HY-P99983, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Pureté et documentation
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Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2) Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2)PD-1/PD-L1PD-1/Programmed death-ligand 1PD-L1B7-H1PD-1D265ACancerInhibitorinhibitorinhibit

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Anti-Mouse PD-L1/B7-H1 (D265A) Antibody (10F.9G2)
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HY-P990172
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