Anti-Mouse TIM-3 Antibody (B8.2C12)
Based on 1 Customer Validation
Anti-Mouse TIM-3 Antibody (B8.2C12) is an anti-mouse TIM-3 IgG1 monoclonal antibody. Anti-Mouse TIM-3 Antibody (B8.2C12) can block the binding of Tim-3 with Phosphatidylserine (PtdSer) and CEACAM1 without interfering with the binding to Galectin-9. Anti-Mouse TIM-3 Antibody (B8.2C12) can inhibit tumor growth and activate tumor infiltrating CD8+ T cells. Anti-Mouse TIM-3 Antibody (B8.2C12) can be used for studying cancer such as breast cancer and colon cancer and constructing experimental autoimmune encephalomyelitis (EAE) models.
For research use only. We do not sell to patients.
- Purity: 99.10%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG1 kappa
Mouse
TIM-3
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL, 7 days) increases Th17 cell differentiation and IL-17A production in CD4+CD25- T cells from the spleen of C57/BL6 mice[2].
Anti-Mouse TIM-3 Antibody (B8.2C12) (4 μg/mL, 3 days) promotes CD4+ T cells apoptosis, but has no significant effect on CD8+ T cells from the spleen of experimental autoimmune encephalomyelitis (EAE) mice[2].
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL) can block the binding of Tim-3 and PtdSer in thymocyte from Balb/c mice and the binding of Tim-3 to CEACAM1 in Jurkat T cells[3].
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL) does not block the binding of Tim-3 to mouse or human Galectin-9[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CD4+ T cells and CD8+ T cells
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Concentration:4 μg/mL
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Incubation Time:3 days
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Result:Reduced the proportion of CD4+ T cells.
Had no significant effect on the proportion of CD8+ T cells.
Anti-Mouse TIM-3 Antibody (B8.2C12) (300 μg, i.v., from day 8 post-immunization, for 5 days) successfully constructed the experimental autoimmune encephalomyelitis (EAE) model in C57/BL6 mice[2].
Anti-Mouse TIM-3 Antibody (B8.2C12) (200 μg, i.p., once every 3 days, for a total of 2 shots) may exert anti-tumor effects by blocking ligand binding or downregulating Tim-3 in wild type Balb/c mice bearing CT26 tumors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5×104 4T1 cells injected female BALB/c mice (6 weeks)[1]
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Dosage:100 μg, combined with ETO (50 mg/kg, i.v., on day 6 and 18)
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Administration:Intraperitoneal injection (i.p.), on day 7, 12, and 17
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Result:Significantly inhibited tumor volume.
Significantly reduced cancer stem cell (CSC) frequency.
Downregulated the level of STT3 in tumor cells.
Significantly increased CD8+ IFN-γ+ T cells.
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Animal Model:C57/BL6 mice (7-8 weeks)[2]
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Dosage:300 μg, combined with MOG35-55 (HY-P3719)
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Administration:Intravenous injection (i.v.), from day 8 post-immunization for 5 days
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Result:Increased the proportion of CD4+ T cells and Th17 cells in the spinal cord.
Aggravated the symptoms of EAE.
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Animal Model:1×106 CT26 cells injected wild type Balb/c mice[3]
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Dosage:200 μg
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Administration:Intraperitoneal injection (i.p.), once every 3 days for a total of 2 shots
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Result:Did not alter the frequency of Treg cells within the tumor.
Might downregulate Tim-3 and exert anti-tumor effects.
Q8VIM0-1
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
ELISA, FACS, Functional assay, Research in vivo
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse TIM-3 Antibody (B8.2C12)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Hsu JM, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun. 2018 May 15;9(1):1908. [Content Brief]
[2]. Dema M, et al. IL-6 Inhibition as a Therapeutic Target in Aged Experimental Autoimmune Encephalomyelitis. Int J Mol Sci. 2024 Jun 19;25(12):6732. [Content Brief]
[3]. Sabatos-Peyton CA, et al. Blockade of Tim-3 binding to phosphatidylserine and CEACAM1 is a shared feature of anti-Tim-3 antibodies that have functional efficacy. Oncoimmunology. 2017 Nov 9;7(2):e1385690. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse TIM-3 Antibody (B8.2C12) Related Classifications
HY-P990004 Related Classifications
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