Anti-pan-Glypican Antibody (HS20)

Cat. No.: HY-P990841 Purity: 99.44%: Data Sheet
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Anti-pan-Glypican Antibody (HS20) is a kind of human IgG1 κ human antibody, targeting to human pan-Glypican. Anti-pan-Glypican Antibody (HS20) can neutralize the heparan sulfate (HS) chains on GPC3 thereby disrupting the Wnt3a-GPC3 interactions leading to blockade of the Wnt3a/β-catenin signaling. Anti-pan-Glypican Antibody (HS20) can be used for the research of cancer, such as hepatocellular carcinoma (HCC).

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Anti-pan-Glypican Antibody (HS20) 화학구조

사이즈	재고	수량
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

pan-Glypican

In Vitro

Anti-pan-Glypican Antibody (HS20) specifically recognizes the heparan sulfate (HS) chains on GPC3, also recognizes other glypicans (GPC1, GPC5 and GPC6)^[1].
Anti-pan-Glypican Antibody (HS20) (10-50 μg/mL, 2 h) blocks blocks the interaction of Wnt3a and GPC3 and blocks Wnt3a-induced accumulation of active β-catenin in HEK293-SuperTopFlash cells^[2].
Anti-pan-Glypican Antibody (HS20) (25-100 μg/mL, 2 h) inhibits Wnt-dependent cell proliferation in Hep3B and Huh-7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-pan-Glypican Antibody (HS20) (20 mg/kg, i.v., three times a week) inhibits tumor growth in Hep3B/HepG2 tumor mice (BALB/c nu/nu nude mice)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Hep3B/HepG2 tumor mice (4-6 weeks, female, BALB/c nu/nu nude mice)^[1]
Dosage:	20 mg/kg
Administration:	Intravenously injection, three times a week
Result:	Inhibited tumor growth. Had less β-catenin staining and contained fewer proliferating cells.

Application

in vivo blocking of Wnt signaling; in vitro blocking of Wnt signaling; Functional assays; ELISA;

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

분자량

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-pan-Glypican Antibody (HS20)]

선적

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Purity: 99.44%

Select Batch:

Data Sheet (260 KB) SDS (251 KB)

COA (232 KB) SDS-PAGE (143 KB) SEC-HPLC (105 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. Gao W, et al. Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate. Sci Rep. 2016 May 17;6:26245. [Content Brief]

[2]. Gao W, et al. Inactivation of Wnt signaling by a human antibody that recognizes the heparan sulfate chains of glypican-3 for liver cancer therapy. Hepatology. 2014 Aug;60(2):576-87. [Content Brief]

Anti-pan-Glypican Antibody (HS20) Related Classifications

몰농도 계산기
농도 희석 계산기

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-pan-Glypican Antibody (HS20)Glycoprotein VIGPVIGPC3GPC1GPC5GPC6Wnt3aHepatocellular carcinomaHep3BHuh-7HepG2Inhibitorinhibitorinhibit

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