ABCA1 Antibody (YA3573)(PBS only)

Cat. No.: HY-P83876A: Data Sheet User Guide for Antibodies
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ABCA1 Antibody (YA3573) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ABCA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

ABCA1 Antibody (YA3573) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ABCA1.

Host

Mouse

Molecular Weight

Predicted band size: 254 kDa;

Observed band size: 254 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit, Monkey

SwissProt ID

O95477

Gene ID

19 [NCBI]

Immunogen

Purified recombinant fragment of human ABCA1 (AA: 2081-2261) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Endosome

Expression：
Tissue_specificity:Widely expressed, but most abundant in macrophages

Induction:By bacterial lipopolysaccharides (LPS) . LPS regulates expression through a liver X receptor (LXR) -independent mechanism. Repressed by ZNF202

Subunit：Interacts with MEGF10 (PubMed:17205124). May interact with APOE1; functionally associated with APOE1 in the biogenesis of HDLs (PubMed:14754908). Interacts with ABCA8; this interaction potentiates cholesterol efflux (PubMed:28882873). Interacts with ABCA12 and NR1H2; this interaction is required for ABCA1 localization to the cell surface and is necessary for its normal activity and stability (PubMed:23931754)

Synonyms

TGD; ABC1; CERP; ABC-1; HDLDT1; HPALP1; HDLCQTL13

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hep-2-cell.html [Content Brief]