ATP6V1B1 Antibody (YA7871)(PBS only)

Cat. No.: HY-P88187A: Data Sheet User Guide for Antibodies
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ATP6V1B1 Antibody (YA7871) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP6V1B1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

ATP6V1B1 Antibody (YA7871) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP6V1B1.

Host

Mouse

Molecular Weight

Predicted band size: 56.7 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P15313

Immunogen

Full length human recombinant protein of human ATP6V1B1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:16769747). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). Essential for the proper assembly and activity of V-ATPase (PubMed:16769747). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed:16769747). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (By similarity)

Subcellular Localization：Apical cell membrane,Basolateral cell membrane

Expression：
Tissue_Specificity: Kidney; localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level) (PubMed:16769747, PubMed:29993276). Expressed in the cochlea and endolymphatic sac (PubMed:9916796)

Isoforms & Post-Translational Modification：P15313: 513 amino acids, molecular weight 56833 Da.

Subunit：V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (By similarity)

Synonyms

ATP6B1; RTA1B; VATB; VMA2; VPP3

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/vero-cell.html [Content Brief]