Bak Antibody (YA4312)(PBS only)

Cat. No.: HY-P84615A: Data Sheet User Guide for Antibodies
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Bak Antibody (YA4312) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Bak.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Bak Antibody (YA4312) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Bak.

Host

Mouse

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q16611

Gene ID

578 [NCBI]

Immunogen

Purified recombinant fragment of human BAK1 (AA: 29-187) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases

Subcellular Localization：Mitochondrion outer membrane; Single-pass membrane protein

Expression：
Tissue_specificity:This gene is expressed in a variety of tissues, with the highest expression levels in the heart and skeletal muscle.

Isoforms & Post-Translational Modification：Q16611 has 2 isomers: Q16611-1: 23409 Da (predicted); Q16611-2: 16872 Da (predicted).

Subunit：Homodimer. Formation of the homodimer is zinc-dependent (PubMed:17157251). Forms heterodimers with BCL2 and BCL2L1 isoform Bcl-X(L) (PubMed:9020082). Forms heterooligomers with BAX (PubMed:29531808). Interacts with BCL2A1 (By similarity). Interacts with RTL10/BOP (PubMed:23055042). Interacts with VDAC1 (PubMed:25296756). Interacts with GIMAP3/IAN4 and GIMAP5/IAN5 (PubMed:16509771)

Synonyms

BAK; CDN1; BCL2L7; BAK-LIKE

Documentation

User Guide for Antibodies (1077 KB)