CBLC Antibody (YA8585)(PBS only)

Cat. No.: HY-P88901A: Data Sheet User Guide for Antibodies
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CBLC Antibody (YA8585) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBLC.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CBLC Antibody (YA8585) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBLC.

Host

Mouse

Molecular Weight

Predicted band size: 52.3 kDa

Species Reactivity

Human

SwissProt ID

Q9ULV8

Immunogen

Human recombinant protein fragment corresponding to amino acids 244-474 of human CBLC produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：Q9ULV8 has two isomers: Q9ULV8-1: 52456 Da (predicted); Q9ULV8-2: 47418 Da (predicted).
Phosphorylated on multiple tyrosine residues by SRC丨Autoubiquitinated when phosphorylated at Tyr-341, enhanced by SRC; suggesting proteasomal degradation

Subunit：Interacts with ubiquitin-conjugating enzyme E2 UBE2D2 and UBE2D3

Synonyms

CBL-3; CBL-SL; RNF57

Documentation

User Guide for Antibodies (1077 KB)