CBWD1 Antibody (YA8437)

Cat. No.: HY-P88753: Data Sheet User Guide for Antibodies
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CBWD1 Antibody (YA8437) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CBWD1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CBWD1 Antibody (YA8437) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CBWD1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 43.9 kDa

Species Reactivity

Human, Dog, Rat, Monkey

SwissProt ID

Q9BRT8

Immunogen

Full length human recombinant protein of human CBWD1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Zinc chaperone that directly transfers zinc cofactor to target metalloproteins, thereby activating them. Catalyzes zinc insertion into the active site of methionine aminopeptidase METAP1, which function to cleave the initiator methionine from polypeptides during or after protein translation. Mechanistically, the N-terminal psi-PxLVp motif binds to the C6H2-type zinc finger of inactive form of METAP1. After formation of the docked complex, zinc is transferred from the CXCC motif in the GTPase domain of ZNG1A to the zinc binding site in the peptidase domain of METAP1 in a process requiring GTP hydrolysis. GTP/GDP exchange is required for release of active METAP1

Subcellular Localization：Nucleus

Expression：
Tissue_Specificity: Ubiquitously expressed. Up-regulated in cultured astrocytes treated with dopamine
Induction: Down-regulated in response to zinc: repressed by ZNF658 in response to zinc by binding to the zinc transcriptional regulatory element (ZTRE) (5'-C[AC]C[TAG]CC[TC]-N(0-50)-[GA]G[ATC]G[TG]G-3') found in the promoter region of CBWD1 (PubMed:22902622, PubMed:25582195). Increased expression in response to dopamine treatment (PubMed:11489251)

Isoforms & Post-Translational Modification：Q9BRT8 has four isomers: Q9BRT8-1: 44068 Da (predicted); Q9BRT8-2: 40420 Da (predicted); Q9BRT8-3: 42015 Da (predicted); Q9BRT8-4: 12172 Da (predicted).

Synonyms

COBP

Documentation

User Guide for Antibodies (1077 KB)