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CD315 Antibody (YA4544)

(Synonyms: PTGFRN; FPRP; EWI-F; CD9P-1; SMAP-6)
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Based on 1 Customer Validation

CD315 Antibody (YA4544) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD315.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD315 Antibody (YA4544)
  • CD315 Antibody (YA4544) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CD315 Antibody (YA4544) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD315.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 99 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 99 kDa
Immunogen

Purified recombinant fragment of human CD315 (AA: extra 46-221) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD315 Antibody (YA4544)
    Flow cytometric analysis of 1X10^6 A431 cells labeling CD315 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD315 inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. Functional coupling with the prostaglandin F2-alpha receptor seems to occur. In myoblasts, associates with tetraspanins CD9 and CD81 to prevent myotube fusion during muscle regeneration

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein

  • Subunit

    Interacts with CD9 and CD81 (PubMed:11278880). Also seems to interact with CD63, CD82 and CD151 (PubMed:11278880)

  • SwissProt ID

    Q9P2B2

  • Gene ID
    5738 [NCBI]
  • Synonyms

    PTGFRN; FPRP; EWI-F; CD9P-1; SMAP-6

CD315 Antibody (YA4544) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A431 cells labeling CD315 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD315 Antibody (YA4544) Product Image
    1/16
  • Experimental Validation Results for CD315 Antibody (YA4544)
  • CD315 Antibody (YA4544) Product Image
  • Experimental Validation Results for CD315 Antibody (YA4544)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A431 cells labeling CD315 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD315 Antibody (YA4544)
  • CD315 Antibody (YA4544) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 A431 cells labeling CD315 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD315 Antibody (YA4544) Product Image FC