CLEC10A/CD301 Antibody (YA7116)(PBS only)

Cat. No.: HY-P87428A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

CLEC10A/CD301 Antibody (YA7116) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CLEC10A/CD301.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CLEC10A/CD301 Antibody (YA7116) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CLEC10A/CD301.

Host

Mouse

Molecular Weight

Predicted band size: 35.4kDa;

Species Reactivity

Human

SwissProt ID

Q8IUN9

Immunogen

Purified recombinant fragment of human CD301 (AA: extra 158-316) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：C-type lectin receptor involved in recognition of N-acetylgalactosamine (GalNAc)-terminated glycans by myeloid antigen presenting cells (APCs) (PubMed:15802303, PubMed:16998493, PubMed:17616966, PubMed:22213806, PubMed:33724805, PubMed:8598452). Binds in a Ca(2+)-dependent manner to alpha- and beta-linked GalNAc residues on glycoprotein and glycolipid antigens, including alphaGalNAc- and Galbeta1->3GalNAc-O-Ser/Thr also known as Tn and T antigens, LacdiNAc epitope GalNAcbeta1->4GlcNAc and its derivative GalNAcbeta1->4-(Fucalpha1->3)GlcNAc, O-linked core 5 and 6 glycans, and GM2 and GD2 gangliosides (PubMed:15802303, PubMed:23507963). Acts as a signaling receptor at the interface of APC-T cell interactions. On immature dendritic cells, recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and downregulates PTRPN/CD45 phosphatase activity with an impact on T cell activation threshold, cytokine production and proliferation. Modulates dendritic cell maturation toward a tolerogenic phenotype leading to generation of regulatory CD4-positive T cell subset with immune suppressive functions (PubMed:15802303, PubMed:16998493, PubMed:22213806). Acts as an endocytic pattern recognition receptor involved in antitumor immunity. During tumorigenesis, recognizes Tn antigens and its sialylated forms Neu5Ac-Tn and Neu5Gc-Tn expressed on tumor cell mucins. On immature dendritic cells, can internalize Tn-terminated immunogens and target them to endolysosomal compartment for MHC class I and II antigen presentation to CD8-positive and CD4-positive T cells, respectively (PubMed:15802303, PubMed:17616966, PubMed:17804752)

Subcellular Localization：Cell membrane,Early endosome membrane,Lysosome membrane

Expression：
Tissue_Specificity: Expressed in myeloid antigen presenting cells in lymph nodes and skin (at protein level). Expressed in dermal dendritic cells (at protein level)

Isoforms & Post-Translational Modification：Q8IUN9 has three isomers: Q8IUN9-1: 35446 Da (predicted); Q8IUN9-2: 32884 Da (predicted); Q8IUN9-3: 28877 Da (predicted).

Subunit：Interacts with A-, B- and C-domain containing PTPRC/CD45 isoforms: isoform 1/CD45ABC, isoform 3/CD45AB, isoform 5/CD45BC and isoform 7/CD45B

Synonyms

CD301, CLECSF13, CLECSF14, HML, CLEC10A, C-type lectin domain family 10 member A, C-type lectin superfamily member 14, Macrophage lectin 2

Documentation

User Guide for Antibodies (1077 KB)