EIF5A2 Antibody (YA8484)(PBS only)

Cat. No.: HY-P88800A: Data Sheet User Guide for Antibodies
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EIF5A2 Antibody (YA8484) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EIF5A2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

EIF5A2 Antibody (YA8484) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EIF5A2.

Host

Mouse

Molecular Weight

Predicted band size: 16.6 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9GZV4

Immunogen

Full length human recombinant protein of human EIF5A2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed:14622290). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (By similarity)

Subcellular Localization：Cytoplasm,Nucleus,Endoplasmic reticulum membrane

Expression：
Tissue_Specificity: Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells

Isoforms & Post-Translational Modification：Q9GZV4: 153 amino acids, molecular weight 16793 Da.

Subunit：Binds to 80S ribosomes (By similarity)

Synonyms

EIF-5A2; eIF5AII

Documentation

User Guide for Antibodies (1077 KB)