GPR56 Antibody (YA4598)(PBS only)

Cat. No.: HY-P84901A: Data Sheet User Guide for Antibodies
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GPR56 Antibody (YA4598) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GPR56.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GPR56 Antibody (YA4598) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GPR56.

Host

Mouse

Molecular Weight

Predicted band size: 78 kDa;

Observed band size: 55-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y653

Gene ID

9289 [NCBI]

Immunogen

Purified recombinant fragment of human GPR56 (AA: extra(26-225)) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Adhesion G-protein coupled receptor (aGPCR) for steroid hormone 17alpha-hydroxypregnenolone (17-OH), which is involved in cell adhesion and cell-cell interactions (PubMed:39389061). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as RhoA pathway (PubMed:28874577, PubMed:35418682, PubMed:39389061). ADGRG1 is coupled to G(12) and/or G(13) G proteins (GNA12 and GNA13, respectively) and mediates the activation Rho small GTPases (PubMed:22238662, PubMed:28424266, PubMed:35418682, PubMed:39389061). Acts as a potent suppressor of ferroptosis: binding to 17-OH-binding initiates signaling that down-regulates CD36 and alleviates ferroptosis-induced liver injury (By similarity). Ligand-binding also induces cell adhesion activity via association with proteins such as collagen III/COL3A1 and TGM2 (By similarity). Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells (By similarity). Involved in cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination: association with COL3A1 in the developing brain inhibits neuronal migration via activation of the RhoA pathway (PubMed:24531968). Together with TGM2, acts as a regulator of myelination and myelin repair in oligodendrocyte precursor cells (By similarity). Acts as a hemostatic sensor of shear force: G protein-coupled receptor signaling is activated in response to shear force in platelets, promoting G(13) G protein signaling, and platelet shape change and aggregation in a COL3A1-dependent manner (PubMed:33097663). Acts as an inhibitor of VEGFA production thereby inhibiting angiogenesis through a signaling pathway mediated by PRKCA (PubMed:16757564, PubMed:19572147, PubMed:21724588). Plays a role in the maintenance of hematopoietic stem cells in bone marrow niche (By similarity). Plays an essential role in testis development (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Secreted; Membrane raft

Expression：
Tissue_specificity:It is widely distributed, with the highest concentrations in the thyroid, brain, and heart. It is expressed in a large number of tumor cells. Its expression is downregulated in highly metastatic tumor cells.

Isoforms & Post-Translational Modification：Q9Y653 has 5 isomers: Q9Y653-1: 77738 Da (predicted); Q9Y653-2: 77072 Da (predicted); Q9Y653-3: 77476 Da (predicted); Q9Y653-4: 58655 Da (predicted); Q9Y653-5: 58042 Da (predicted).
Autoproteolytically cleaved into 2 fragments; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1 CT) predominantly remain associated and non-covalently linked (PubMed:22333914, PubMed:37673336). Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s) (PubMed:22333914);N-glycosylated. Contains sialic acid residues;Ubiquitinated. Undergoes polyubiquitination upon activation

Subunit：Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1-CT) predominantly remain associated and non-covalently linked (PubMed:22333914). ADGRG1 NT self-associates in a trans-trans manner; the homophilic interaction enhances receptor signaling (PubMed:21708946). Interacts with TGM2 (PubMed:16757564, PubMed:21349848, PubMed:21724588). Interacts with heparin; leading to the reduction of ADGRG1 shedding (PubMed:27068534). Interacts with COL3A1 (PubMed:28258187). Part of a GPCR-tetraspanin complex at least consisting of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of ADGRG1 with GNA11 (PubMed:15004227)

Synonyms

BFPP; BPPR; ADGRG1; TM7LN4; TM7XN1

Documentation

User Guide for Antibodies (1077 KB)