GSPT2 Antibody (YA8520)

Cat. No.: HY-P88836: Data Sheet User Guide for Antibodies
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GSPT2 Antibody (YA8520) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GSPT2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GSPT2 Antibody (YA8520) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GSPT2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 68.7 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8IYD1

Immunogen

Full length human recombinant protein of human GSPT2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed:11524954, PubMed:15987998, PubMed:17562865). GSPT2/ERF3B mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:15987998). GTP hydrolysis by GSPT2/ERF3B induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed:15987998). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:19417104)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Highly expressed in IUCC stage II colorectal cancer (CRC)

Isoforms & Post-Translational Modification：Q8IYD1: 628 amino acids, molecular weight 68883 Da.

Subunit：Component of the eRF1-eRF3-GTP ternary complex, composed of ETF1/ERF1 and ERF3 (GSPT1/ERF3A or GSPT2/ERF3B) and GTP (PubMed:15987998)

Synonyms

ERF3B; GST2

Documentation

User Guide for Antibodies (1077 KB)