GSPT2 Antibody (YA8520)
(Synonyms: ERF3B; GST2)GSPT2 Antibody (YA8520) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GSPT2.
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Host:
Mouse
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Isotype:
IgG1
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Application:
ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:100-250 | 1:100 |
Product Details
GSPT2 Antibody (YA8520) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GSPT2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 68.7 kDa
Full length human recombinant protein of human GSPT2 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GSPT2 is a GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT2/ERF3B mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT2/ERF3B induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons[1][2][3][4].
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Subcellular Localization
Cytoplasm
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Expression
Tissue_Specificity: Highly expressed in IUCC stage II colorectal cancer (CRC) -
Isoforms & Post-Translational Modification
Q8IYD1: 628 amino acids, molecular weight 68883 Da.
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Subunit
Component of the eRF1-eRF3-GTP ternary complex, composed of ETF1/ERF1 and ERF3 (GSPT1/ERF3A or GSPT2/ERF3B) and GTP (PubMed:15987998)
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SwissProt ID
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Synonyms
ERF3B; GST2
Documentation
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