HNF 4 alpha Antibody (YA4004)
(Synonyms: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha)Based on 1 Customer Validation
HNF 4 alpha Antibody (YA4004) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to HNF 4 alpha.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
HNF 4 alpha Antibody (YA4004) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to HNF 4 alpha.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 53 kDa
Purified recombinant fragment of human HNF4A (AA: 1-150) expressed in HEK293-6e cells supernatant.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HNF 4 alpha transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells[1][2].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
P41235 has 7 isomers: P41235-1: 52785 Da (predicted); P41235-2: 51619 Da (predicted); P41235-3: 46623 Da (predicted); P41235-4: 56139 Da (predicted); P41235-5: 50150 Da (predicted); P41235-6: 48984 Da (predicted); P41235-7: 43988 Da (predicted).
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA;Acetylation at Lys-458 lowers transcriptional activation by about two-fold -
Subunit
Homodimerization is required for HNF4-alpha to bind to its recognition site (PubMed:14982928). Interacts with CLOCK, BMAL1, CRY1, CRY2, PER1 and PER2 (PubMed:30530698). Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionally inactive (PubMed:28128295). Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers (PubMed:28128295)
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SwissProt ID
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Synonyms
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha
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