INCENP Antibody (YA6960)(PBS only)
(Synonyms: binds and activates aurora B and C in vivo and in vitro antibody; Chromosomal passenger protein antibody; INCE_HUMAN antibody; INCENP antibody; Inner centromere protein antibody; Inner centromere protein antigens 135/155kDa antibody; Inner centromere protein antigens 135kD 155kD antibody; Inner centromere protein INCENP antibody)INCENP Antibody (YA6960) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to INCENP.
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Host:
Mouse
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Isotype:
IgG1
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Application:
ICC/IF, IHC-P, FC
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
INCENP Antibody (YA6960) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to INCENP.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 105.4 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 105.4 kDa
Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.
Protein A affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
INCENP component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin. Activates AURKB and AURKC. Required for localization of CBX5 to mitotic centromeres. Controls the kinetochore localization of BUB1[1][2][3][4][5].
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Subcellular Localization
Nucleus,Chromosome, centromere,Cytoplasm, cytoskeleton, spindle,Midbody,Chromosome, centromere, kinetochore
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Isoforms & Post-Translational Modification
Q9NQS7 has two isomers: Q9NQS7-1: 105429 Da (predicted); Q9NQS7-2: 104992 Da (predicted).
Phosphorylation by AURKB or AURKC at its C-terminal part is important for AURKB or AURKC activation by INCENP -
Subunit
Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex binds directly to AURKB or AURKC via the IN box, and forms a triple-helix bundle-based subcomplex with BIRC5 and CDCA8 via its N-terminus (PubMed:17956729, PubMed:27332895)
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SwissProt ID
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Synonyms
binds and activates aurora B and C in vivo and in vitro antibody; Chromosomal passenger protein antibody; INCE_HUMAN antibody; INCENP antibody; Inner centromere protein antibody; Inner centromere protein antigens 135/155kDa antibody; Inner centromere protein antigens 135kD 155kD antibody; Inner centromere protein INCENP antibody
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