INCENP Antibody (YA6960)(PBS only)

Cat. No.: HY-P87277A: Data Sheet User Guide for Antibodies
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INCENP Antibody (YA6960) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to INCENP.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

INCENP Antibody (YA6960) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to INCENP.

Host

Mouse

Molecular Weight

Predicted band size: 105.4 kDa;

Observed band size: 105.4 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NQS7

Immunogen

Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	Protein A affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed:12925766, PubMed:15316025, PubMed:27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed:27332895). Required for localization of CBX5 to mitotic centromeres (PubMed:21346195). Controls the kinetochore localization of BUB1 (PubMed:16760428)

Subcellular Localization：Nucleus,Chromosome, centromere,Cytoplasm, cytoskeleton, spindle,Midbody,Chromosome, centromere, kinetochore

Isoforms & Post-Translational Modification：Q9NQS7 has two isomers: Q9NQS7-1: 105429 Da (predicted); Q9NQS7-2: 104992 Da (predicted).
Phosphorylation by AURKB or AURKC at its C-terminal part is important for AURKB or AURKC activation by INCENP

Subunit：Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex binds directly to AURKB or AURKC via the IN box, and forms a triple-helix bundle-based subcomplex with BIRC5 and CDCA8 via its N-terminus (PubMed:17956729, PubMed:27332895)

Synonyms

binds and activates aurora B and C in vivo and in vitro antibody; Chromosomal passenger protein antibody; INCE_HUMAN antibody; INCENP antibody; Inner centromere protein antibody; Inner centromere protein antigens 135/155kDa antibody; Inner centromere protein antigens 135kD 155kD antibody; Inner centromere protein INCENP antibody

Documentation

User Guide for Antibodies (1077 KB)