Ki67 Antibody (YA001)

Cat. No.: HY-P80734: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Ki67 Antibody (YA001) is a Rat-derived and non-conjugated IgG2b monoclonal antibody, targeting to Ki67.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Ki67 Antibody (YA001) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Ki67 Antibody (YA001) is a Rat-derived and non-conjugated IgG2b monoclonal antibody, targeting to Ki67.

Host

Rat

Clonality

Monoclonal

Molecular Weight

Predicted band size: 359 kDa;

Observed band size: 359 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P46013

Gene ID

4288 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Ki67.AA range:1816-2163.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in phosphate buffered saline (pH 7.4) and150 mM NaCl. Preservative: 0.02% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Ki67Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Ki67Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Isoforms & Post-Translational Modification：P46013 has 2 isomers: P46013-1: 358694 Da (predicted); P46013-2: 319444 Da (predicted).
Hyperphosphorylated by CDK1 in mitosis; hyperphosphorylatiom prevents undergoing liquid-liquid phase separation (PubMed:10502411, PubMed:10653604, PubMed:25012651, PubMed:39153474). Dephosphorylated by PPP1CC at the onset of anaphase (PubMed:25012651). Dephosphorylated by protein phosphatase 2A (PP2A) at the onset of anaphase (By similarity). Dephosphorylation by protein phosphatase 2A (PP2A) and simultaneous exposure of the positively charged patch (CP) during mitotic exit induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface (PubMed:39153474);Ubiquitinated by the APC/C complex after neuronal progenitors exit mitosis during brain development, leading to clearance from constitutive heterochromatin

RRID

AB_3102683

Database

Entrez Gene: 4288 Human ; 17345 Mouse ; 246042 Rat

SwissProt: P46013 Human ; E9PVX6 Mouse ; Q61769 Mouse ;

OMIM: 176741 Human

Research Field

Cell Biology

Synonyms

MKI67; Antigen KI-67

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Ki67 Antibody (YA001) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Ki67 Antibody (YA001)

Ki67 Antibody (YA001) Featured Recommendations:

Product Detail

Verification Image

Background

Documentation

Ki67 Antibody (YA001) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report